Description

Folinic Acid Calcium Salt Pentahydrate CAS NO 41927-89-3 is the calcium salt form of folinic acid (leucovorin), a vital biochemical used as an active pharmaceutical ingredient and a key intermediate in advanced synthesis. This compound matters for its role in modulating folate metabolism, serving as a rescue agent in chemotherapy and a critical component in cell culture media formulations. It is primarily needed by pharmaceutical manufacturers, biotechnology research facilities, and fine chemical producers developing oncology treatments, nutritional supplements, and high-purity biochemical reagents.

Application

• As an active pharmaceutical ingredient (API) in the formulation of leucovorin calcium injections and oral medications.
• As a chemotherapy rescue agent to counteract the toxic effects of methotrexate and other folate antagonist drugs in oncology.
• As a critical supplement in cell culture media and fermentation processes for biotechnology and pharmaceutical production.
• In the manufacture of high-purity biochemical reagents and reference standards for analytical and diagnostic applications.
• As a key intermediate in the synthesis of more complex folate analogs and related pharmaceutical compounds.
• For nutritional and clinical research involving folate metabolism, DNA synthesis, and cell proliferation studies.

Basic Information

Product Name	Folinic Acid Calcium Salt Pentahydrate
CAS No.	41927-89-3
Molecular Formula	C20H21CaN7O7 • 5H2O
Molecular Weight	601.59 g/mol (Pentahydrate)
Synonyms	Leucovorin Calcium Pentahydrate; Calcium Folinate Pentahydrate; 5-Formyltetrahydrofolate Calcium Salt; (6S)-5,10-Methenyltetrahydrofolate Calcium Salt; Citrovorum Factor Calcium Salt; NSC-3590; Antifolan; Wellcovorin (Brand Name); (2S)-2-[[4-[[(6S)-2-Amino-5-formyl-4-oxo-1,6,7,8-tetrahydro-6-pteridinyl]methyl]amino]benzoyl]amino]pentanedioic Acid Calcium Salt Pentahydrate
EINECS	255-599-7

Quality Control

Our Folinic Acid Calcium Salt Pentahydrate is manufactured under strict quality systems, with specifications designed to meet or exceed relevant pharmacopoeial standards such as USP and EP. Each batch undergoes rigorous analytical testing including HPLC for assay and purity, identification by IR and UV, and tests for specific impurities and residual solvents. A comprehensive Certificate of Analysis (COA) documenting all test results is provided with every shipment to ensure full traceability and compliance.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and must be kept in a dry environment to prevent degradation. For long-term storage, consider desiccated conditions.

Specification

Item	Specification
Appearance	White to slightly yellow crystalline powder
Identification (IR)	Conforms to reference spectrum
Identification (UV)	Conforms
Assay (HPLC, anhydrous basis)	97.0% - 102.0%
Water Content (Karl Fischer)	13.0% - 16.0%
pH (1% Solution)	6.5 - 8.5
Specific Optical Rotation	+14.0° to +20.0°
Related Substances (HPLC)	Total impurities ≤ 2.0% Any individual impurity ≤ 1.0%
Residual Solvents (GC)	Complies with ICH guidelines
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.