Description

4-Bromo-Calcium Ionophore A23187 Free Acid is a chemically modified derivative of the well-known divalent cation ionophore A23187. This compound is specifically engineered for advanced research applications, offering enhanced properties for probing calcium signaling pathways and membrane transport mechanisms. It is a critical tool for biochemists, pharmacologists, and cell biologists working in pharmaceutical R&D, academic research, and diagnostic assay development.

Application

• Biochemical Research: A key reagent for studying intracellular calcium (2+) flux, signaling cascades, and ion channel function in cell-free and cellular systems.
• Pharmaceutical Development: Used in high-throughput screening (HTS) assays to identify and characterize novel drug candidates targeting calcium-dependent processes.
• Cell Physiology Studies: Facilitates the manipulation of cytosolic calcium levels to investigate its role in apoptosis, muscle contraction, neurotransmitter release, and enzyme activation.
• Fluorescent Probe Conjugation: The bromo moiety provides a reactive site for coupling with fluorescent dyes, creating custom imaging probes for live-cell microscopy.
• Membrane Biophysics: Serves as a model ionophore for studying the kinetics and thermodynamics of ion transport across lipid bilayers and artificial membranes.
• Diagnostic Assay Kits: Incorporated as a core component in commercial and research-grade kits for measuring calcium concentrations in biological samples.

Basic Information

Product Name	4-Bromo-Calcium Ionophore A23187 Free Acid
CAS No.	76455-82-8
Molecular Formula	C29H37BrN2O6
Molecular Weight	589.53 g/mol
Synonyms	4-Bromo-A23187; 4-Bromo Calcimycin; 4-Br-A23187; 4-Bromoantibiotic A23187; 4-Bromo-5-(methylamino)-2-[[(1R,2R,3S,5R)-2,3,5-trimethyl-6-oxocyclohexyl]methyl]benzoic acid; Brominated Calcium Ionophore; 4-Bromo free acid of A23187
EINECS	Contact for details

Quality Control

Every batch of 4-Bromo-Calcium Ionophore A23187 Free Acid is manufactured and tested under strict quality management protocols. We employ advanced analytical techniques including HPLC, NMR, and mass spectrometry to ensure high purity and structural identity. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles is provided with each shipment to support your research and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a dry environment before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.