Description

Phorbol-13-Acetate-12-n-Methyl-n-4-(N,N'-Di(2-Hydroxyethyl)Amino)-7-Nitrobenz-2-Oxa-1,3-Diazole-Aminododecanoate is a highly specialized, fluorescently-labeled derivative of phorbol ester, designed for advanced biochemical research. This compound is critical for studying protein kinase C (PKC) signaling pathways with high spatial and temporal resolution due to its integrated NBD (7-nitrobenz-2-oxa-1,3-diazole) fluorophore. It is an essential tool for researchers and pharmaceutical developers in cell biology, neuroscience, and oncology, enabling precise visualization and quantification of PKC activation and translocation in live cells.

Application

• Live-Cell Imaging Probes: Used as a fluorescent tracer to visualize real-time activation and subcellular localization of Protein Kinase C (PKC) isoforms in living systems.
• Mechanistic Pharmacology Studies: Employed to investigate the mechanisms of PKC activators and inhibitors, aiding in drug discovery for cancer and neurological disorders.
• Signal Transduction Research: A key reagent for studying downstream effects of PKC-mediated pathways in cell proliferation, differentiation, and apoptosis.
• High-Content Screening (HCS): Serves as a vital component in automated imaging assays to identify novel modulators of PKC activity for therapeutic development.
• Biomarker Development: Utilized in developing diagnostic assays that correlate PKC activation states with specific disease phenotypes.
• Academic & Institutional Research: Fundamental tool for universities and research institutes exploring cellular signaling mechanisms.

Basic Information

Product Name	Phorbol-13-Acetate-12-n-Methyl-n-4-(N,N'-Di(2-Hydroxyethyl)Amino)-7-Nitrobenz-2-Oxa-1,3-Diazole-Aminododecanoate
CAS No.	137034-69-6
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	NBD-Phorbol-12,13-Diacetate; 12-N-Methyl-N-(4-(N,N-di(2-hydroxyethyl)amino)-7-nitrobenz-2-oxa-1,3-diazol-4-yl)dodecanamido-13-acetate Phorbol; Phorbol 12,13-Diacetate NBD Conjugate; 4-(N,N-Di(2-hydroxyethyl)amino)-7-nitrobenz-2-oxa-1,3-diazole-labeled Phorbol Ester; Fluorescent Phorbol Ester; PKC Fluorescent Activator; NBD-PDBu analog; NBD-C12-phorbol-13-acetate
EINECS	Contact for details

Quality Control

Every batch of our fluorescent phorbol ester is produced and analyzed under strict quality control protocols to ensure the highest standards of purity and performance for sensitive research applications. We guarantee identity, purity, and fluorescence integrity through comprehensive analytical techniques including HPLC, NMR, and mass spectrometry. A detailed Certificate of Analysis (COA), documenting purity, spectral data, and functional performance, is provided with each shipment to support your research and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) to prevent oxidation. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Orange to red solid or film
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Fluorescence Emission	Conforms to expected profile
Solubility	Soluble in DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.