Description

5 5'-Difluor-Bapta-Am is a specialized, high-purity fluorinated derivative of BAPTA (1,2-bis(o-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid), a well-known calcium chelator. This compound is engineered for advanced research applications requiring precise modulation of intracellular calcium signaling with enhanced photostability and membrane permeability. It is primarily utilized by researchers and developers in the fields of cell biology, neuroscience, and fluorescence probe design, where reliable and high-quality chemical tools are critical for experimental success.

Application

• Fluorescent Calcium Indicator Precursor: Key intermediate in the synthesis of next-generation, ratiometric fluorescent dyes (e.g., Fura-2 analogs) for intracellular calcium imaging.
• Neurobiological Research: Used to develop probes for studying calcium dynamics in neurons, synaptic transmission, and cellular signaling pathways.
• Cell Physiology Studies: Essential for creating tools to investigate calcium's role in muscle contraction, cell division, apoptosis, and other fundamental processes.
• Diagnostic Reagent Development: Serves as a building block for diagnostic assays and kits designed to detect and quantify calcium flux in clinical and research settings.
• Photostable Probe Design: The difluoro modification enhances photostability, making it valuable for developing probes for long-term or high-intensity imaging experiments.
• Chemical Biology Tool: Used to create cell-permeable chelators for selectively buffering or clamping intracellular calcium concentrations.

Basic Information

Product Name	5 5'-Difluor-Bapta-Am
CAS No.	128255-42-5
Molecular Formula	C22H20F2N2O10
Molecular Weight	510.40 g/mol
Synonyms	5,5'-Difluoro-BAPTA-AM; 1,2-Bis(2-amino-5-fluorophenoxy)ethane-N,N,N',N'-tetraacetic acid tetrakis(acetoxymethyl ester); 5,5'-Difluoro-1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid acetoxymethyl ester; BAPTA-AM, 5,5'-difluoro-; Difluoro BAPTA AM; AM ester of 5,5'-Difluoro BAPTA; Cell-permeable difluoro BAPTA; [Ca2+] indicator precursor
EINECS	Contact for details

Quality Control

Our 5 5'-Difluor-Bapta-Am is manufactured under strict quality control protocols to ensure batch-to-batch consistency and high purity, essential for sensitive research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to ISO 9001 quality management standards, and our production environment is designed to meet the rigorous demands of life science research.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). For long-term storage, consider desiccation and under an inert atmosphere to maintain stability and purity. Allow the sealed vial to equilibrate to room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Heavy Metals	≤ 20 ppm
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.