Description

Cytotoxic Necrotizing Factor Type 1 (CNF1) is a potent bacterial protein toxin primarily produced by certain strains of Escherichia coli. This product is critical for advanced biomedical research, particularly in the study of cellular signaling pathways, cytoskeleton dynamics, and the mechanisms of bacterial pathogenesis. It is an essential reagent for researchers and developers in the fields of cell biology, immunology, and toxicology, facilitating the investigation of Rho GTPase activation and its role in disease processes.

Application

• Cell Biology Research: Used as a tool to study Rho GTPase activation, actin cytoskeleton reorganization, and cell morphology changes.
• Toxicology Studies: Employed to investigate the mechanisms of bacterial virulence and host-cell intoxication.
• Signal Transduction Analysis: Serves as a specific activator of Rho, Rac, and Cdc42 proteins to dissect downstream signaling cascades.
• Pharmaceutical Development: Utilized in target validation and screening assays for novel anti-infective or anti-inflammatory therapeutics.
• Academic & Institutional Research: A key reagent for universities and research institutes focusing on microbiology and host-pathogen interactions.
• Biotechnology Reagent Production: Supplied as a high-purity, characterized protein for inclusion in research kits and assay systems.

Basic Information

Product Name	Cytotoxic Necrotizing Factor Type 1
CAS No.	106803-33-2
Molecular Formula	Contact for details
Molecular Weight	~110 kDa (Monomer)
Synonyms	CNF1; CNF-1; Cytotoxic Necrotizing Factor 1; E. coli Cytotoxic Necrotizing Factor Type 1; Dermonecrotic Toxin; Rho-activating Toxin
EINECS	Contact for details

Quality Control

Our Cytotoxic Necrotizing Factor Type 1 is produced and tested under stringent conditions to ensure high biological activity and purity, suitable for sensitive research applications. Each batch is characterized for identity, purity, and functional activity. Certificates of Analysis (COA) detailing purity (by SDS-PAGE), endotoxin levels, and specific biological activity are provided to guarantee batch-to-batch consistency and compliance with research-grade standards.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized material at -20°C or below. Reconstituted solutions should be aliquoted and stored at ≤ -70°C to prevent repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the container is sealed under inert atmosphere or desiccant after each use.

Specification

Item	Specification
Appearance	White or off-white lyophilized powder
Identification (SDS-PAGE)	Corresponds to standard
Purity (SDS-PAGE)	≥ 90%
Protein Content (BCA)	≥ 95% of total
Biological Activity (Cell Rounding Assay)	Defined EC50 per batch
Endotoxin (LAL)	< 1.0 EU/µg
Solubility	Soluble in aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.