Description

H-Lys-Ala-Amc Hcl is a synthetic peptide derivative, specifically a fluorogenic substrate designed for enzymatic activity assays. This compound is critical for biochemical research and pharmaceutical development, enabling precise measurement of protease and peptidase activity. It is primarily utilized by research institutions, diagnostic kit manufacturers, and pharmaceutical companies involved in drug discovery and proteomics.

Application

• Protease/Peptidase Research: As a fluorogenic substrate for studying enzyme kinetics, inhibition, and specificity of various proteolytic enzymes.
• High-Throughput Screening (HTS): Used in automated drug discovery platforms to identify and characterize potential therapeutic enzyme inhibitors.
• Diagnostic Assay Development: A key component in the formulation of sensitive and specific clinical diagnostic tests for enzyme-related pathologies.
• Biochemical Pathway Analysis: Employed in cell-based and cell-free systems to elucidate proteolytic signaling pathways and regulatory mechanisms.
• Quality Control in Biologics: Used to monitor and quantify residual protease activity during the purification and manufacturing of biopharmaceuticals.

Basic Information

Product Name	H-Lys-Ala-Amc Hcl
CAS No.	103404-62-2
Molecular Formula	C19H28N5O4 • HCl
Molecular Weight	426.92 g/mol
Synonyms	L-Lysyl-L-alanine 7-amido-4-methylcoumarin hydrochloride; Lys-Ala-AMC • HCl; Nα-(7-Amino-4-methylcoumarin-3-yl)-L-lysyl-L-alanine hydrochloride; H-Lys-Ala-AMC hydrochloride; Ala-Lys-AMC • HCl; Fluorogenic substrate Lys-Ala-AMC; Protease substrate 103404-62-2; L-Lysine, N2-[N-(7-amino-4-methyl-2-oxo-2H-1-benzopyran-3-yl)-L-alanyl]-, hydrochloride (1:1)
EINECS	Contact for details

Quality Control

Our H-Lys-Ala-Amc Hcl is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and stringent controls for residual solvents and moisture. Certificates of Analysis (COA) detailing all specifications are provided to ensure traceability and compliance with your research or production requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, desiccate at low temperature. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Peptide Content	≥ 70.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.