Description

Exoenzyme C3 is a highly specific bacterial enzyme, also known as C3 transferase, with the CAS registry number 58319-92-9. This product is a critical research tool for studying and modulating Rho GTPase signaling pathways, particularly the inactivation of RhoA, RhoB, and RhoC. It is essential for researchers and developers in the fields of cell biology, neuroscience, and cardiovascular research who require a reliable, high-purity reagent for investigating cytoskeletal dynamics, cell adhesion, and migration.

Application

• Cell Biology Research: Used as a specific inhibitor of RhoA/B/C GTPases to study actin cytoskeleton organization, cell morphology, and cell motility.
• Neuroscience Studies: Applied in research on axon guidance, growth cone collapse, and neuronal regeneration by modulating Rho signaling pathways.
• Cardiovascular Research: Investigated for its role in vascular smooth muscle contraction, endothelial barrier function, and the pathophysiology of hypertension.
• Oncology Research: Utilized to explore the role of Rho GTPases in cancer cell invasion, metastasis, and tumor progression.
• Drug Discovery & Development: Serves as a reference standard and tool compound for screening and validating novel therapeutics targeting Rho-mediated pathways.
• Biochemical Assay Development: Employed in the development of enzymatic assays to measure ADP-ribosylation activity and screen for modulators.

Basic Information

Product Name	Exoenzyme C3
CAS No.	58319-92-9
Molecular Formula	C~270H~420N~84O~80S~2 (Approx.)
Molecular Weight	~25-28 kDa
Synonyms	C3 Exoenzyme; C3 Transferase; ADP-ribosyltransferase C3; Clostridium botulinum C3 exoenzyme; Rho-inactivating C3 transferase; EC 2.4.2.-; Botulinum C3 exoenzyme; C3bot; C3 exotoxin; C3-like ADP-ribosyltransferase
EINECS	Contact for details

Quality Control

Our Exoenzyme C3 is produced under stringent conditions to ensure batch-to-batch consistency and high biological activity. Each lot undergoes rigorous quality testing, including assessment of purity, enzymatic activity, and endotoxin levels. A comprehensive Certificate of Analysis (COA) detailing identity, purity, specific activity, and sterility (where applicable) is provided with every shipment to support your research and regulatory needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized material at -20°C or below. Reconstituted solutions should be aliquoted and stored at -70°C to -80°C to minimize freeze-thaw cycles. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use in a low-humidity environment.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (SDS-PAGE)	Single band at ~25-28 kDa
Purity (HPLC)	≥ 95%
Specific Activity	≥ 1.0 x 10⁴ units/mg
Protein Content (UV)	≥ 90%
Endotoxin Level (LAL)	< 1.0 EU/µg
Solubility	Soluble in aqueous buffers
Biological Activity (Cell-based)	Induces morphological changes in Rho-expressing cells

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.