Description

A23187 is a potent, calcium-selective ionophore widely used as a biochemical tool to manipulate intracellular calcium levels. This compound is critical for researchers studying calcium-mediated signaling pathways, cellular physiology, and ion transport mechanisms. It serves as an essential reagent for the pharmaceutical, biotechnology, and life science research industries, enabling studies in cell biology, neurobiology, and cardiology.

Application

• Cell Biology Research: Used to artificially raise cytosolic calcium concentrations to study calcium's role as a second messenger in processes like muscle contraction, neurotransmitter release, and gene expression.
• Pharmacology & Toxicology: Employed as a research tool to investigate calcium-dependent enzyme activity, cell viability, and the mechanisms of drugs that interact with calcium channels.
• Mitochondrial Function Studies: Facilitates the study of calcium uptake and release in mitochondria, which is crucial for understanding cellular energy metabolism and apoptosis.
• Secretion Studies: Used to trigger calcium-dependent exocytosis in cells such as neurons, endocrine cells, and immune cells to study secretory pathways.
• Fluorescence-Based Assays: Acts as a positive control in high-throughput screening assays that utilize calcium-sensitive fluorescent dyes (e.g., Fluo-4, Fura-2).
• Ion Channel Research: A key tool for distinguishing between store-operated calcium entry (SOCE) and other calcium influx pathways in electrophysiology and cell signaling experiments.

Basic Information

Product Name	A23187
CAS No.	52665-69-7
Molecular Formula	C29H37N3O6
Molecular Weight	523.62 g/mol
Synonyms	Calcimycin; Antibiotic A23187; Calcium Ionophore A23187; 4-Bromo-A23187 (common derivative); (2S,3S,5R,6S,8R,9R,11E)-5-Methyl-6-[(2R,3R,5S)-5-methyl-3,6-dioxo-5-propanyl-2-piperazinyl]-2,3,8,9-tetrahydro-8,9-dihydroxy-2,3-dimethyldodeca-4,6,11-trien-1,7-dione; 5-(Methylamino)-2-[[(2S,3R,5R,6S,8R,9R)-3,6,8,9-tetrahydro-2,8,9-trihydroxy-3,6-dimethyldecahydro-2H-1,7-dioxacycloundecin-5-yl]oxy]-4-(2-oxopropyl)-4,6-dimethyl-2H-1-benzopyran-7-carboxylic acid methyl ester
EINECS	258-101-5

Quality Control

Our A23187 is produced under strict quality management systems to ensure batch-to-batch consistency and reliability for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting identity, purity, and relevant physicochemical parameters.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. For long-term storage, solutions of A23187 should be prepared in anhydrous DMSO or ethanol and kept at -80°C under an inert atmosphere to prevent degradation. Allow the product to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.5%
Solubility	Soluble in DMSO, DMF, ethanol; insoluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.