Description

Cpf4, Pde-Fret-Sensor CAS NO 407578-87-4 is a specialized biochemical sensor compound designed for fluorescence resonance energy transfer (FRET) assays targeting phosphodiesterase (PDE) activity. This high-purity reagent is critical for enabling precise, real-time monitoring of enzymatic kinetics and cyclic nucleotide signaling in complex biological systems. It is an essential tool for pharmaceutical R&D, academic research, and diagnostic development in the life sciences sector, particularly for drug discovery programs focused on neurological, cardiovascular, and inflammatory pathways.

Application

• High-Throughput Screening (HTS): Core component in FRET-based assays for identifying and characterizing novel phosphodiesterase (PDE) inhibitors in drug discovery pipelines.
• Enzyme Kinetics Studies: Used to measure real-time PDE activity, providing detailed kinetic parameters (Km, Vmax) for mechanistic research.
• Cell-Based Signaling Research: Enables the visualization and quantification of intracellular cyclic nucleotide dynamics (e.g., cAMP, cGMP) in live cells.
• Diagnostic Assay Development: Serves as a key reagent in the development of clinical and research diagnostic kits for conditions involving PDE dysregulation.
• Academic & Institutional Research: Fundamental tool for studying cell signaling pathways in neuroscience, cardiology, and immunology.
• Biomarker Validation: Supports research into PDE isoforms as potential therapeutic targets or disease biomarkers.

Basic Information

Product Name	Cpf4, Pde-Fret-Sensor
CAS No.	407578-87-4
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	PDE FRET Sensor Cpf4; Cpf4 cAMP Sensor; Phosphodiesterase Fluorescence Sensor; FRET-based PDE Activity Probe; Cyclic Nucleotide FRET Probe; PDE Activity Assay Substrate; 407578-87-4; Cpf4 PDE Inhibitor Screening Reagent
EINECS	Contact for details

Quality Control

Every batch of our Cpf4, Pde-Fret-Sensor is manufactured under strict quality management systems and undergoes comprehensive analytical testing to ensure high purity, batch-to-batch consistency, and optimal performance in sensitive FRET applications. Our quality commitment includes verification of spectral properties, functional activity, and the absence of interfering impurities. A detailed Certificate of Analysis (COA) documenting identity, purity (HPLC), and functional assay data is provided with each shipment to support your research and regulatory needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This compound is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to minimize condensation and moisture uptake. For long-term stability, store desiccated.

Specification

Item	Specification
Appearance	Lyophilized powder or solid
Identification (IR / NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Functional Activity (FRET Assay)	Conforms to reference standard
Extinction Coefficient	Provided on COA
Fluorescence Properties	Excitation/Emission maxima provided on COA
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, buffer solutions

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.