Description

G418 CAS NO 49863-47-0 is a potent aminoglycoside antibiotic widely used as a selective agent in molecular biology and genetic engineering. Its primary value lies in its ability to effectively inhibit protein synthesis in eukaryotic cells, making it an indispensable tool for stable cell line selection. This product is essential for researchers and manufacturers in the biotechnology, pharmaceutical, and life sciences industries who require reliable and high-purity selection reagents for genetic studies and bioproduction.

Application

• Selection agent in mammalian cell culture for generating stable cell lines expressing recombinant genes.
• Critical component in genetic engineering workflows, including the selection of transfected cells with plasmids containing the neo resistance gene.
• Research tool in molecular biology for studying gene function, expression, and regulation in eukaryotic systems.
• Used in the development and production of biopharmaceuticals, such as monoclonal antibodies and recombinant proteins, using engineered cell lines.
• Application in basic research involving stem cells, cancer biology, and other areas requiring precise genetic manipulation.
• Quality control and cell line maintenance in industrial bioprocessing and contract research organizations (CROs).

Basic Information

Product Name	G418
CAS No.	49863-47-0
Molecular Formula	C20H40N4O10
Molecular Weight	496.55 g/mol
Synonyms	Geneticin; G418 Sulfate; G418 Disulfate; Antibiotic G 418; NSC 305263; 4,6-Dideoxy-4-[[2,6-dideoxy-2-(1,3-dihydroxy-2-propylamino)-α-D-ribo-hexopyranosyl]amino]-α-D-glucopyranosyl]amino]-2-hydroxycyclohexyl] 2-deoxy-D-streptamine sulfate; Antibiotic G418; G-418 Sulfate
EINECS	256-516-3

Quality Control

Our G418 is produced under strict quality management systems to ensure batch-to-batch consistency and high biological activity. Each lot undergoes comprehensive analytical testing, including HPLC for purity and potency, sterility testing, and endotoxin level analysis. A detailed Certificate of Analysis (COA) is provided with each shipment, confirming compliance with our stringent specifications for research and bioproduction use.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. The product is hygroscopic (moisture-sensitive). Keep the container tightly sealed in a dry environment to maintain stability and potency.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms
Assay (HPLC)	≥98.0% (anhydrous basis)
pH (1% solution)	4.0 - 6.0
Water Content (KF)	≤5.0%
Endotoxin	<10 EU/mg
Biological Activity	≥750 µg/mg
Sterility (Membrane Filtration)	Sterile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.