Description

Geneticin (G418 Sulfate) is a potent aminoglycoside antibiotic widely used as a selective agent in molecular biology and cell culture. Its core value lies in enabling stable, long-term selection of genetically modified eukaryotic cells that express neomycin resistance genes. This product is essential for researchers and bioprocessing professionals in biotechnology, pharmaceutical development, and academic institutions who require reliable and high-purity selection reagents for genetic engineering workflows.

Application

• Stable Cell Line Selection: Primary application for selecting mammalian, plant, yeast, and other eukaryotic cells transfected with neomycin (neor) resistance genes.
• Genetic Engineering & Transfection: Critical for establishing and maintaining genetically engineered cell pools expressing recombinant proteins or gene knockouts/knock-ins.
• Biopharmaceutical Production: Used in the development and manufacturing of cell lines for monoclonal antibody and therapeutic protein production.
• Gene Function Studies: Enables selection in functional genomics screens, CRISPR-Cas9 editing validation, and other research requiring stable genetic modification.
• Virus Packaging Cell Lines: Employed in the selection and maintenance of packaging cell lines used for producing recombinant viral vectors.
• Microbial Selection: Can be used for selecting certain bacteria, fungi, or protozoa engineered with aminoglycoside resistance markers.

Basic Information

Product Name	Geneticin (G418 Sulfate)
CAS No.	108321-42-2
Molecular Formula	C20H40N4O10 • H2SO4
Molecular Weight	692.71 g/mol
Synonyms	G418 Sulfate; G418 Disulfate; Geneticin Selective Antibiotic; Antibiotic G 418; NSC 305263; Geneticin sulfate salt; 2-Deoxystreptamine derivative antibiotic; O-2-Amino-2-deoxy-α-D-glucopyranosyl-(1→4)-O-[3-deoxy-4-C-methyl-3-(methylamino)-β-L-arabinopyranosyl-(1→6)]-2-deoxy-N3-ethyl-D-streptamine sulfate
EINECS	Contact for details

Quality Control

Our Geneticin (G418 Sulfate) is manufactured under strict quality systems to ensure batch-to-batch consistency and performance in sensitive cell culture applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and potency, verification of selective activity in cell-based assays, and testing for endotoxin and bioburden levels. A detailed Certificate of Analysis (COA) is provided with every shipment, confirming compliance with our stringent specifications.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent absorption of moisture, which can affect stability and potency.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to reference spectrum
Assay (HPLC)	≥98.0% (anhydrous basis)
pH (1% Solution)	4.0 - 6.0
Water Content (KF)	≤5.0%
Residue on Ignition	≤1.0%
Biological Activity	Meets cell culture selection performance criteria
Endotoxin	<1.0 EU/mg

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.