Description

1,2-Dioleoyloxy-3-(Trimethylammonium)Propane is a cationic lipid widely used as a key transfection reagent component. This compound is critical for facilitating the delivery of nucleic acids and other macromolecules into cells for research and therapeutic development. It is primarily needed by biotechnology, pharmaceutical, and academic research sectors focused on gene therapy, vaccine development, and advanced cellular studies.

Application

• Lipid Nanoparticle (LNP) Formulation: A fundamental cationic lipid component for constructing stable LNPs used in mRNA vaccine and therapeutic delivery systems.
• Non-Viral Gene Transfection: Serves as a critical reagent in liposome-based transfection kits for introducing DNA, siRNA, or mRNA into mammalian cells in vitro.
• Drug Delivery Vehicle: Used to create cationic liposomes for the targeted delivery of small molecule drugs, proteins, and other bioactive agents.
• Biomedical Research: Essential tool for molecular and cell biology research involving gene expression studies, protein production, and functional genomics.
• Therapeutic Development: Employed in pre-clinical development of gene therapies and nucleic acid-based treatments for various diseases.

Basic Information

Product Name	1,2-Dioleoyloxy-3-(Trimethylammonium)Propane
CAS No.	113669-21-9
Molecular Formula	C44H86NO4+
Molecular Weight	692.18 g/mol (as chloride salt)
Synonyms	DOTAP; 1,2-Dioleoyl-3-trimethylammonium-propane; DOTAP Chloride; N-[1-(2,3-Dioleoyloxy)propyl]-N,N,N-trimethylammonium chloride; TAP Lipid; CL-1; Cationic lipid 1; (2,3-Dioleoyloxy-propyl)-trimethylammonium chloride
EINECS	Contact for details

Quality Control

Our 1,2-Dioleoyloxy-3-(Trimethylammonium)Propane (DOTAP) is manufactured under strict quality systems. Each batch undergoes comprehensive analytical testing including HPLC purity assay, identity confirmation (NMR, MS), and impurity profiling to ensure consistency and suitability for sensitive biomedical applications. Certificates of Analysis (COA) with full traceability are provided.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) to prevent oxidation and hydrolysis. The product is hygroscopic; allow the sealed vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white waxy solid or viscous liquid
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Residual Solvents (GC)	Meets ICH guidelines
Water Content (KF)	≤ 1.0%
Phase Transition Temperature (DSC)	Approx. -10°C to 0°C

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.