Description

Hygromycin B CAS NO 31282-04-9 is an aminoglycoside antibiotic produced by *Streptomyces hygroscopicus*. This compound is critically important as a selective agent in molecular biology and cell culture for the stable maintenance of plasmids carrying the hygromycin resistance gene. It is an essential reagent for researchers and manufacturers in the biotechnology and pharmaceutical industries, particularly for the selection of genetically engineered prokaryotic and eukaryotic cells.

Application

• Selection Agent in Molecular Biology: Used to select and maintain mammalian, plant, bacterial, and yeast cells transfected with vectors containing the hygromycin B phosphotransferase (hph) gene.
• Stable Cell Line Development: A critical component in the creation of stable recombinant cell lines for biopharmaceutical production and research.
• Antibiotic in Microbiology: Exhibits activity against a broad spectrum of bacteria, fungi, and higher organisms, finding use in specific microbiological studies.
• Veterinary and Agricultural Research: Historically used as an anthelmintic and for growth promotion in swine and poultry; research applications in parasitology and transgenic plant selection continue.
• Gene Function Studies: Enables functional genomics research by allowing for the stable integration and expression of genes of interest in various host systems.

Basic Information

Product Name	Hygromycin B
CAS No.	31282-04-9
Molecular Formula	C20H37N3O13
Molecular Weight	527.52 g/mol
Synonyms	Hygromycin; Hygromix; Hygromisin; O-De(2-amino-2-deoxy-α-D-glucopyranosyl)-5-deoxy-3-C-[(1S)-(1,3-dihydroxy-2-propyl)-2-O-β-D-talopyranosyl-D-chiro-inositol]; Antibiotic XK-44; Antibiotic 172; NSC 529634; Streptomyces hygroscopicus antibiotic
EINECS	250-531-4

Quality Control

Our Hygromycin B is produced and tested under a rigorous quality management system to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot is analyzed for potency, purity, and the absence of endotoxins. A comprehensive Certificate of Analysis (COA) detailing specifications such as HPLC purity, biological potency, pH, and endotoxin levels is provided with every shipment to support your research and quality documentation requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive). For long-term storage, desiccate. Keep container tightly sealed when not in use to prevent absorption of atmospheric moisture.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to reference standard
Assay (HPLC)	≥ 95.0%
Potency	≥ 600 µg/mg
pH (1% Solution)	6.0 - 8.0
Water Content (KF)	≤ 5.0%
Endotoxin	< 10 EU/mg
Biological Activity	Confirmed by cell culture assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.