Description

Phorbol 12,13-Dibutyrate is a potent, cell-permeable diacylglycerol (DAG) analog and a highly specific activator of protein kinase C (PKC). This compound is a critical research tool for studying intracellular signal transduction pathways, cellular proliferation, and tumor promotion mechanisms. It is essential for researchers and scientists in the fields of pharmacology, oncology, and molecular biology, serving as a standard in biochemical assays and experimental models.

Application

• Biochemical Research: A standard agonist for activating Protein Kinase C (PKC) isoforms in cell-based and cell-free assays to study signal transduction.
• Cancer Research: Used to investigate tumor promotion mechanisms, cell proliferation, and apoptosis in various cancer cell lines.
• Pharmacological Studies: Employed as a tool compound to evaluate the efficacy of novel PKC inhibitors and modulators in drug discovery.
• Neuroscience: Applied in studies of neurotransmitter release, synaptic plasticity, and neuronal differentiation where PKC signaling is involved.
• Immunology: Used to examine the role of PKC in immune cell activation, cytokine production, and inflammatory responses.
• Reference Standard: Serves as a high-purity analytical standard for quality control and method development in pharmaceutical and research laboratories.

Basic Information

Product Name	Phorbol 12,13-Dibutyrate
CAS No.	37558-16-0
Molecular Formula	C36H56O8
Molecular Weight	616.83 g/mol
Synonyms	PDBu; Phorbol 12,13-Dibutanoate; 12,13-Dibutyrylphorbol; 4,9,12-beta,13,20-Pentahydroxytiglia-1,6-dien-3-one 12,13-dibutyrate; TPA analog; Phorbol 12,13-dibutyrate; 12-O-Tetradecanoylphorbol-13-acetate analog
EINECS	Contact for details

Quality Control

Our Phorbol 12,13-Dibutyrate is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods, to ensure it meets the high standards required for research applications. A Certificate of Analysis (COA) detailing purity, identity, and other critical parameters is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry, cool place. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, keep desiccated under an inert atmosphere. Allow the vial to warm to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%
Solubility	Soluble in DMSO, ethanol, acetone; insoluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.