Description

Poly-L-Ornithine Hydrochloride is a synthetic, positively charged polyamino acid derived from the amino acid ornithine. This compound is essential for creating stable, positively charged surfaces that promote the adhesion of various cell types, including neurons and epithelial cells. It is a critical reagent for researchers and manufacturers in the life sciences, biotechnology, and pharmaceutical industries, particularly for cell culture and tissue engineering applications.

Application

• Cell Culture Substrate Coating: Widely used to coat glass, plastic, and other surfaces to enhance the attachment and growth of anchorage-dependent cells, such as primary neurons, fibroblasts, and stem cells.
• Neuroscience Research: Serves as a standard substrate for culturing primary neuronal cells, facilitating studies in neurodevelopment, neurotoxicity, and synaptic function.
• Tissue Engineering Scaffolds: Employed as a component in biomaterial scaffolds to improve cell seeding efficiency and tissue integration.
• Biomedical Device Coatings: Applied to implantable devices or diagnostic tools to create bio-adhesive surfaces that improve biocompatibility.
• Polyelectrolyte Multilayer Films: Used in conjunction with negatively charged polymers (e.g., poly-L-glutamic acid) to build layer-by-layer (LbL) films for controlled drug release or surface modification.
• Virus and Gene Delivery Studies: Acts as a transfection enhancer or a component in non-viral gene delivery systems due to its cationic nature.

Basic Information

Product Name	Poly-L-Ornithine Hydrochloride
CAS No.	36630-89-4
Molecular Formula	(C5H12N2O2 • HCl)n
Molecular Weight	Contact for details (Polymer)
Synonyms	Poly(ornithine hydrochloride); PLO; Poly-L-Ornithine HBr (related salt); Poly(ornithine hydrobromide); L-Ornithine homopolymer hydrochloride; Polyornithine; (S)-Poly(aminopropylamino)carboxylic acid hydrochloride; NSC 354731
EINECS	Contact for details

Quality Control

Our Poly-L-Ornithine Hydrochloride is produced under stringent quality management systems. Each batch undergoes comprehensive analytical testing to ensure identity, purity, and performance consistency suitable for sensitive cell culture applications. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting key parameters such as molecular weight distribution, endotoxin levels, and sterility (where applicable).

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder or lyophilized solid
Identification (IR)	Conforms to structure
Assay (Nitrogen)	≥ 95.0%
Molecular Weight Range	15,000 - 70,000 Da (Typical)
Endotoxin	< 1.0 EU/mg (for cell culture grade)
Solubility	Soluble in water
pH (1% solution)	5.0 - 7.0

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.