Description

Ptdins-(3,4,5)-P3-Biotinamide (Sodium Salt) is a high-purity, biotin-conjugated derivative of the critical secondary messenger phosphatidylinositol (3,4,5)-trisphosphate (PIP3). This compound is essential for advanced biochemical research, enabling the specific detection, isolation, and study of PIP3-binding proteins and signaling complexes. It is primarily utilized by researchers and developers in academic institutions, pharmaceutical R&D, and biotechnology companies focused on cell signaling, lipidomics, and drug discovery for oncology and metabolic diseases.

Application

• Biochemical Research Probe: Used as a critical tool for studying phosphoinositide 3-kinase (PI3K) signaling pathways, protein-lipid interactions, and intracellular signal transduction mechanisms.
• Affinity Purification & Pull-Down Assays: The biotin tag facilitates the isolation and identification of PIP3-binding proteins (e.g., PDK1, Akt) using streptavidin-coated beads for proteomic analysis.
• Lipid Kinase/Phosphatase Activity Assays: Serves as a substrate or product analog in enzymatic assays to screen for modulators of PI3K, PTEN, or SHIP phosphatases.
• Membrane Biology Studies: Employed in model membrane systems (e.g., liposomes, lipid bilayers) to investigate the role of PIP3 in membrane recruitment, curvature, and domain formation.
• Drug Discovery Target Validation: A key reagent for validating and characterizing novel therapeutics targeting the PI3K/Akt/mTOR pathway, a major target in cancer therapy.
• Diagnostic Development: Potential use in developing diagnostic assays for diseases characterized by dysregulated PI3K signaling.

Basic Information

Product Name	Ptdins-(3,4,5)-P3-Biotinamide (Sodium Salt)
CAS No.	1415684-80-8
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	PIP3-Biotin; Biotin-PIP3; Biotinylated Phosphatidylinositol (3,4,5)-Trisphosphate; Biotin-PtdIns(3,4,5)P3; 1,2-Diacyl-sn-glycero-3-phospho-(1-D-myo-inositol-3,4,5-trisphosphate) Biotinamide; Biotin-conjugated PI(3,4,5)P3; D-myo-Inositol, 1,2-diacyl-sn-glycero-3-phospho-, 3,4,5-trisphosphate, biotinamide derivative
EINECS	Contact for details

Quality Control

This high-value biochemical is produced under strict quality control protocols to ensure batch-to-batch consistency and reliability for sensitive research applications. Each lot is characterized using advanced analytical techniques including HPLC, MS, and NMR to confirm identity, purity, and functional integrity. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive). For long-term storage, consider desiccation and storage under an inert atmosphere. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid or film
Identification (HPLC/MS)	Conforms to reference standard
Assay (HPLC)	≥ 95%
Purity (HPLC)	≥ 95% (Area %)
Solubility	Soluble in chloroform/methanol/water mixtures
Functional Activity (Pull-down)	Tested and confirmed

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.