Description

Kinase (Phosphorylating), Protein Kinase Akt is a highly specific and purified enzyme critical for catalyzing the transfer of phosphate groups to serine/threonine residues on target proteins, a fundamental process in cellular signaling. This product is essential for researchers and developers in the life sciences sector who require reliable, high-activity enzymes for biochemical research and assay development. It is primarily used in pharmaceutical R&D, academic research, and diagnostic kit manufacturing for studying cell proliferation, apoptosis, and metabolic pathways.

Application

• Biochemical Research & Assay Development: Used as a key reagent in in vitro kinase activity assays to study signal transduction mechanisms.
• Drug Discovery & Screening: Employed in high-throughput screening (HTS) platforms to identify and validate novel kinase inhibitors for therapeutic development.
• Cell Signaling Pathway Analysis: Essential for investigating the PI3K/Akt/mTOR pathway, which is implicated in cancer, diabetes, and neurological disorders.
• Diagnostic Kit Manufacturing: Incorporated into ELISA and other immunoassay kits for the detection of phosphorylated protein biomarkers.
• Academic & Pharmaceutical R&D: Supports fundamental research into protein function, post-translational modifications, and cellular response mechanisms.
• Enzyme Characterization Studies: Used to study enzyme kinetics, substrate specificity, and the effects of modulators on kinase activity.

Basic Information

Product Name	Kinase (Phosphorylating), Protein Kinase Akt
CAS No.	191808-15-8
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	AKT Kinase; Protein Kinase B (PKB); RAC Kinase; RAC-PK; c-AKT; v-Akt Murine Thymoma Viral Oncogene Homolog; Serine/Threonine-Protein Kinase Akt; PKB Alpha; AKT1
EINECS	Contact for details

Quality Control

Our Protein Kinase Akt is produced under stringent quality management systems. Each batch undergoes comprehensive analytical testing, including activity assays, purity assessment, and specificity checks, to ensure it meets the high standards required for research and development. Certificates of Analysis (COA) detailing purity, specific activity, and storage buffer composition are available upon request.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at ≤ -20°C in small aliquots to avoid repeated freeze-thaw cycles. The product is typically supplied in a glycerol-containing buffer; ensure it does not freeze completely at -20°C. Handle with care using appropriate laboratory practices.

Specification

Item	Specification
Appearance	Clear, colorless solution
Purity (SDS-PAGE)	≥ 90%
Specific Activity	≥ XX units/mg (Contact for details)
Identification (Activity)	Phosphorylates known substrate (e.g., GSK-3 fusion protein)
Buffer Composition	XX mM Tris-HCl, XX mM NaCl, XX mM DTT, XX% Glycerol, pH X.X
Endotoxin Level	< 1.0 EU/µg (LAL test)
Storage Condition	-20°C ± 5°C

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.