Description

Zeocin is a potent, broad-spectrum aminoglycoside antibiotic derived from Streptomyces tendae, widely used for selection and maintenance of eukaryotic cells stably transfected with the Sh ble resistance gene. Its exceptional stability in cell culture media and consistent, rapid killing kinetics make it a gold-standard reagent for molecular biology and biopharmaceutical R&D workflows. Researchers and biomanufacturers across academic labs, CROs, and therapeutic development facilities rely on high-purity Zeocin to ensure reproducible transfection efficiency and clonal selection fidelity. Zeocin CAS NO 181494-14-4 is supplied as a sterile-filtered solution or lyophilized powder meeting stringent requirements for sensitive cell-based applications.

Application

• Selection and maintenance of mammalian, insect, plant, and fungal cells expressing the Sh ble (zeocin resistance) gene
• Critical reagent in CRISPR/Cas9, lentiviral, and plasmid-based stable cell line development
• Quality control testing in bioprocess development for antibiotic-free media validation
• Functional assay support in high-throughput screening (HTS) platforms requiring reliable genetic selection
• Production of recombinant proteins and monoclonal antibodies in engineered CHO and HEK293 cell lines
• Research use in synthetic biology and gene circuit engineering where orthogonal selection pressure is required

Basic Information

Product Name	Zeocin
CAS No.	181494-14-4
Molecular Formula	C35H60N18O15S
Molecular Weight	1059.03 g/mol
Synonyms	Zeocin™; Phleomycin D1; Bleomycin D1; Sh ble-selective antibiotic; Streptozocin (historical misnomer, not related); Zecin; Zeocin antibiotic; Phleomycin D; Bleomycin analog D1; 181494-14-4
EINECS	Not listed (non-EU notified substance; REACH registration available upon request)

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards. Certificates of Analysis (COA) are available upon request. Each batch is verified for sterility (USP & Ph. Eur. sterility test), potency (cell-based kill assay using CHO-K1 cells), endotoxin levels (<0.1 EU/mg), and identity (HPLC retention time and UV-Vis spectral match). Manufactured under ISO 9001-certified processes with full traceability.

Storage

Preserve in a tightly closed container, protected from light. Store at 2–8 °C for liquid formulations; store lyophilized powder at –20 °C. Due to its hygroscopic nature, minimize exposure to ambient humidity during handling. Avoid repeated freeze-thaw cycles for solutions.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder or clear, colorless to pale yellow solution
Identification (HPLC)	Retention time matches reference standard (±2%)
Identification (UV-Vis)	λmax at 235 nm and 280 nm in water
Assay (by HPLC)	≥90.0% (anhydrous basis)
Related Substances (HPLC)	Single impurity ≤1.0%; Total impurities ≤3.0%
Endotoxins	<0.1 EU/mg
Sterility	Complies with USP <71>
pH (5 mg/mL in water)	5.5–7.0

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.