Description

Caged Atp, Disodium Salt is a photolabile, biologically inactive derivative of adenosine triphosphate (ATP) designed for precise, light-triggered release of ATP in biological systems. This compound matters because it enables researchers to achieve spatiotemporal control over ATP concentration, a critical energy currency and signaling molecule, in complex experimental setups. It is an essential tool for scientists in neurobiology, muscle physiology, cell signaling research, and drug discovery who require controlled initiation of ATP-dependent processes.

Application

• Cellular Physiology Studies: Precise, localized uncaging of ATP to study ion channel gating, receptor activation, and intracellular calcium signaling dynamics.
• Neurobiological Research: Investigation of neurotransmitter release, synaptic plasticity, and neural circuit function via controlled ATP delivery.
• Muscle Contraction Research: Timed initiation of ATP hydrolysis to study the kinetics of myosin-actin interactions and muscle fiber mechanics.
• Enzyme Kinetics & Mechanism: Rapid mixing experiments using flash photolysis to study the transient kinetics of ATP-dependent enzymes like kinases and ATPases.
• Drug Discovery Assays: High-throughput screening platforms where light-triggered ATP release is used to activate or modulate target proteins.
• Metabolic Pathway Analysis: Controlled perturbation of energy metabolism in live cells or isolated organelles to map flux and regulation.

Basic Information

Product Name	Caged Atp, Disodium Salt
CAS No.	171800-68-3
Molecular Formula	C18H22N5O16P3 • 2Na
Molecular Weight	705.29 g/mol (anhydrous basis)
Synonyms	P3-[1-(2-Nitrophenyl)ethyl] adenosine 5'-triphosphate, disodium salt; NPE-caged ATP; 2-Nitrobenzyl-caged ATP; Adenosine 5'-triphosphate, P3-[1-(2-nitrophenyl)ethyl] ester, disodium salt; "Caged" ATP; Photocaged ATP; ATP, caged (2-Nitrophenylethyl); Light-sensitive ATP derivative.
EINECS	Contact for details

Quality Control

Our Caged Atp, Disodium Salt is produced and handled under strict quality protocols to ensure batch-to-batch consistency and biological activity. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance in standard uncaging assays. We adhere to ISO 9001 quality management principles, and our production facilities follow practices suitable for research-grade biochemicals. Certificates of Analysis (COA) are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This product is strictly light-sensitive (protect from all light exposure) and hygroscopic (moisture-sensitive). For long-term stability, store under inert atmosphere or vacuum desiccation. Allow the vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Water Content (Karl Fischer)	≤ 3.0%
Photolytic Release Efficiency	≥ 70% free ATP released upon irradiation
Biological Activity	Inactive in dark; releases active ATP upon photolysis

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.