Description

3H-1,2,3-Triazolo[4,5-d]pyrimidine, 3-[5-O-[bis(4-methoxyphenyl)phenylmethyl]-3-O-[[bis(1-methylethyl)amino](2-cyanoethoxy)phosphino]-2-O-[(1,1-dimethylethyl)dimethylsilyl]-β-D-ribofuranosyl]- is a sophisticated, high-purity nucleoside phosphoramidite derivative, a critical building block in automated oligonucleotide synthesis. Its primary value lies in enabling the precise and efficient incorporation of modified nucleosides into DNA and RNA sequences, which is essential for advanced research and therapeutic development. This compound is indispensable for pharmaceutical R&D teams, diagnostic developers, and academic researchers working on antisense oligonucleotides, siRNA, aptamers, and other nucleic acid-based technologies.

Application

• Solid-Phase Oligonucleotide Synthesis: Serves as a key phosphoramidite monomer for the automated synthesis of oligonucleotides containing modified nucleobases.
• Therapeutic Oligonucleotide Development: Used in the research and production of antisense drugs, siRNA therapeutics, and aptamers for targeted disease treatment.
• Diagnostic Probe Manufacturing: Essential for creating labeled and modified nucleic acid probes used in PCR, qPCR, FISH, and microarray technologies.
• Structural Biology & Biochemistry Research: Enables the study of nucleic acid structure, function, and protein interactions through site-specific incorporation of modifications.
• Synthetic Biology: Facilitates the construction of novel genetic circuits and functional biomaterials with tailored nucleic acid components.
• Next-Generation Sequencing (NGS): Can be utilized in the development of novel sequencing primers and platforms requiring chemically modified nucleotides.

Basic Information

Product Name	3H-1,2,3-Triazolo[4,5-d]pyrimidine, 3-[5-O-[bis(4-methoxyphenyl)phenylmethyl]-3-O-[[bis(1-methylethyl)amino](2-cyanoethoxy)phosphino]-2-O-[(1,1-dimethylethyl)dimethylsilyl]-β-D-ribofuranosyl]-
CAS No.	771494-03-2
Molecular Formula	C48H61N6O8PSi
Molecular Weight	909.10 g/mol
Synonyms	7-Deaza-8-aza-7-iodo-2'-O-TBDMS-5'-O-DMT-dA Phosphoramidite; 7-Deaza-8-aza-7-iodo-dA Phosphoramidite (2'-O-TBDMS, 5'-O-DMT); 7-Iodo-7-deaza-8-aza-dA CE Phosphoramidite; 7-Iodo-7-deaza-8-aza-2'-deoxyadenosine Phosphoramidite; 7-Iodo-7-deaza-8-aza-dA Phosphoramidite; 7-Iodo-imidazo[4,5-d]pyridazine deoxyriboside Phosphoramidite; 7-Iodo-7-deaza-8-aza-2'-O-TBDMS-5'-O-DMT-2'-deoxyadenosine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; DMT-7-Iodo-7-deaza-8-aza-dA(I)-2'-O-TBDMS-3'-CE Phosphoramidite
EINECS	Contact for details

Quality Control

This high-value phosphoramidite is manufactured under strict quality control protocols to ensure batch-to-batch consistency and optimal coupling efficiency in oligonucleotide synthesis. Production adheres to cGMP guidelines where applicable, with comprehensive analytical characterization including HPLC, 31P NMR, and 1H NMR. A detailed Certificate of Analysis (COA) is provided with each batch, confirming identity, purity, and critical performance parameters.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a desiccated environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (31P NMR)	Conforms to reference standard
Purity (HPLC)	≥ 97.0%
Water Content (KF)	≤ 0.5%
Solubility	Clear, colorless solution in anhydrous acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.