Description

5-Bromo-4-Chloro-3-Indolyl-β-D-Galactoside is a chromogenic substrate widely used in molecular biology and diagnostic assays. Its core value proposition lies in its ability to produce an insoluble blue indigo dye upon enzymatic cleavage, providing a clear, visual readout for the detection of β-galactosidase activity. This compound is essential for researchers and manufacturers in the biotechnology, pharmaceutical, and diagnostic industries, particularly for applications in gene expression studies, reporter gene assays, and microbial detection.

Application

• Reporter Gene Assays: As a key substrate for detecting β-galactosidase (lacZ) gene expression in molecular cloning and genetic engineering.
• Microbiological Testing: For the identification and enumeration of coliform bacteria, such as E. coli, in water quality and food safety testing (e.g., in X-Gal containing media).
• Histochemistry & Cell Biology: Used in staining procedures to localize β-galactosidase activity in tissue sections and cultured cells.
• Enzyme Activity Screening: Employed in high-throughput screening (HTS) platforms to identify modulators of β-galactosidase activity for drug discovery.
• Diagnostic Kit Manufacturing: A critical raw material in the production of diagnostic kits and reagents for clinical and research laboratories.
• Plant Biology Research: Applied in studies of gene regulation and promoter activity in transgenic plants.

Basic Information

Product Name	5-Bromo-4-Chloro-3-Indolyl-β-D-Galactoside
CAS No.	7240-90-6
Molecular Formula	C14H15BrClNO6
Molecular Weight	408.63 g/mol
Synonyms	X-Gal; 5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside; BCIG; 4-Chloro-5-bromo-3-indolyl β-D-galactoside; Galactoside indolyl; 3-Indolyl-β-D-galactopyranoside, 5-bromo-4-chloro-; BCI-GAL; β-Galactosidase substrate X-Gal
EINECS	230-625-4

Quality Control

Our 5-Bromo-4-Chloro-3-Indolyl-β-D-Galactoside is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive biological assays. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and related substance profiles is provided with every shipment to guarantee batch-to-batch consistency and traceability.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. The product is light-sensitive and should be handled under appropriate conditions to prevent degradation. For long-term storage, desiccate at -20°C.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Melting Point	Contact for details
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMF, DMSO; slightly soluble in water
Heavy Metals	< 20 ppm
Biological Activity	Suitable for β-galactosidase detection

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.