Description

Sephadex G-15 is a cross-linked dextran gel designed for gel filtration chromatography. This product is essential for the separation and purification of small molecules, peptides, and other low molecular weight compounds in aqueous solutions. It is a critical tool for researchers and process engineers in the biotechnology, pharmaceutical, and life science industries who require reliable, size-based fractionation. The material is supplied as a dry, free-flowing powder, identified by CAS No. 11081-40-6.

Application

• Desalting and Buffer Exchange: Rapid removal of salts, dyes, or other small contaminants from protein, peptide, and nucleic acid samples.
• Fractionation of Biomolecules: Separation of peptides, oligosaccharides, and other compounds with molecular weights below approximately 1500 Da.
• Sample Preparation for Analysis: Clean-up of samples prior to analytical techniques such as HPLC, mass spectrometry, or electrophoresis.
• Pharmaceutical Purification: Downstream processing steps in the purification of synthetic peptides and small molecule APIs.
• Laboratory-Scale Process Development: Optimization of separation protocols in research and development settings.
• Educational and Training Purposes: A standard medium for teaching principles of gel filtration chromatography in academic laboratories.

Basic Information

Product Name	Sephadex G-15
CAS No.	11081-40-6
Molecular Formula	(C6H10O5)n
Molecular Weight	High polymer
Synonyms	Dextran gel G-15; Sephadex® G-15; Cross-linked dextran gel; Gel filtration medium G-15; Gel chromatography medium G-15; Dextran cross-linked with epichlorohydrin; Poly(dextran epichlorohydrin)
EINECS	234-299-6

Quality Control

Our Sephadex G-15 is manufactured under controlled conditions to ensure consistent performance characteristics, including particle size distribution, exclusion limit, and swelling properties. Each batch is tested to meet stringent in-house specifications for chromatographic performance. A Certificate of Analysis (COA) detailing key parameters such as particle size, water regain, and bed volume is provided with every shipment to guarantee reliable and reproducible results in your applications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and must be kept in a low-humidity environment to maintain its free-flowing properties and performance. Once opened, ensure the container is resealed tightly to prevent moisture uptake.

Specification

Item	Specification
Appearance	White, free-flowing powder
Exclusion Limit (Globular Proteins)	~1,500 Da
Water Regain	1.5 ± 0.2 g/g dry gel
Bed Volume	2.5 - 3.5 ml/g dry gel
Particle Size (Dry)	40 - 120 μm
Swelling Time (in water)	3 hours at 20°C
pH Stability (Long-term)	2 - 13
Operating Temperature	4 - 110°C

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.