Description

Sephadex G-150 is a cross-linked dextran gel designed for size-exclusion chromatography, offering a specific fractionation range ideal for separating large biomolecules. This product is essential for researchers and process engineers requiring high-resolution purification of proteins, polysaccharides, and other macromolecules in aqueous systems. It is a critical tool for downstream processing in the biotechnology and pharmaceutical industries, as well as for analytical applications in academic and industrial R&D laboratories.

Application

• Size-Exclusion Chromatography (SEC/GFC): For the separation and purification of high molecular weight proteins, antibodies, viruses, and polysaccharides based on hydrodynamic size.
• Biopharmaceutical Downstream Processing: Used in the polishing and final purification steps of therapeutic proteins, monoclonal antibodies, and vaccines.
• Desalting and Buffer Exchange: Efficient removal of salts, detergents, or other small molecules from protein and nucleic acid samples.
• Molecular Weight Estimation: Calibration of SEC columns to determine the approximate molecular weights of unknown biological polymers.
• Oligonucleotide and Plasmid DNA Purification: Fractionation of nucleic acids and removal of process contaminants.
• Industrial Enzyme Preparation: Purification of enzymes and other macromolecular catalysts for food, beverage, and biofuel production.

Basic Information

Product Name	Sephadex G-150
CAS No.	12774-36-6
Molecular Formula	(C6H10O5)n
Molecular Weight	High Polymer
Synonyms	Dextran gel; Cross-linked dextran; Sephadex® G-150; Gel filtration medium G-150; Dextran polymer cross-linked with epichlorohydrin; Size exclusion chromatography gel; G-150 gel; Gel filtration matrix
EINECS	Contact for details

Quality Control

Our Sephadex G-150 is manufactured under a rigorous quality management system. Each lot is tested to ensure consistent performance characteristics, including particle size distribution, exclusion limits, and swelling properties. While not a simple chemical, quality is assured through specifications for flow rate, bed volume, and molecular fractionation range. Certificates of Analysis (COA) detailing lot-specific performance data are available upon request to support your quality and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at room temperature (15-25°C). This product is hygroscopic (moisture-sensitive). To maintain optimal performance and prevent microbial growth, ensure the container is sealed after use and avoid exposure to high humidity.

Specification

Item	Specification
Product Form	Dry, free-flowing beads
Particle Size (dry)	40 - 120 μm
Fractionation Range (Globular Proteins)	5,000 - 300,000 Da
Exclusion Limit (Globular Proteins)	~300,000 Da
Bed Volume (swollen in water)	15 - 20 mL/g
pH Stability (long term)	2 - 13
Operating pH Range	2 - 10
Maximum Linear Flow Rate	10 - 15 cm/h

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.