Description

Thymidine, [Methyl-14C] is a carbon-14 radiolabeled nucleoside essential for tracing and quantifying DNA synthesis and cellular proliferation. This compound is critical for providing definitive, quantitative data in metabolic and pharmacokinetic studies, offering unparalleled specificity for tracking thymidine incorporation. It is a fundamental tool for researchers in pharmaceutical development, oncology research, and molecular biology who require precise radiotracers for autoradiography, liquid scintillation counting, and in vitro cell proliferation assays.

Application

• DNA Synthesis & Cell Proliferation Assays: Used as a tracer to measure rates of DNA synthesis in cell cultures and tissues.
• Pharmacokinetics & ADME Studies: Tracks the absorption, distribution, metabolism, and excretion of novel nucleoside-based therapeutics.
• Oncology & Cancer Research: Investigates tumor cell growth dynamics and evaluates the efficacy of anti-cancer drugs that target DNA replication.
• Molecular Biology Research: Employed in pulse-chase experiments and studies of DNA repair mechanisms.
• Autoradiography: Enables precise visualization and localization of DNA synthesis in histological samples.
• Biochemical Pathway Analysis: Traces the metabolic fate of thymidine in salvage and de novo synthesis pathways.

Basic Information

Product Name	Thymidine, [Methyl-14C]
CAS No.	13123-07-4
Molecular Formula	C₁₀H₁₄¹⁴CN₂O₅
Molecular Weight	Contact for details
Synonyms	1-(2-Deoxy-β-D-ribofuranosyl)-5-methyl[methyl-¹⁴C]uracil; [Methyl-¹⁴C]Thymidine; Thymidine-methyl-¹⁴C; 2'-Deoxy[methyl-¹⁴C]thymidine; dT-[methyl-¹⁴C]; Radiolabeled Thymidine; Carbon-14 Labeled Thymidine; DNA Synthesis Tracer
EINECS	Contact for details

Quality Control

Every batch of Thymidine, [Methyl-14C] is manufactured and tested under strict quality systems to ensure high chemical, radiochemical, and isotopic purity. Our products undergo rigorous quality testing to ensure compliance with industry standards. Certificates of Analysis (COA) detailing specific activity, purity by HPLC, radiochemical purity, and solvent content are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at or below -20°C. This product is strictly light-sensitive (protect from all light exposure). Follow all applicable laboratory safety and radiation protection guidelines for handling and disposal.

Specification

Item	Specification
Appearance	White to off-white solid or solution (specified)
Identification (IR/HPLC)	Conforms to reference standard
Specific Activity	Contact for details (mCi/mmol)
Assay (HPLC)	≥ 98% (Chemical Purity)
Radiochemical Purity (HPLC)	≥ 97%
Solvent (if supplied as solution)	Specified (e.g., Ethanol:Water)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.