Description

Digoxigenin is a high-purity, naturally occurring cardenolide aglycone derived from the foxglove plant, serving as a critical biochemical intermediate and labeling reagent. Its primary commercial value lies in its role as the core hapten for generating highly specific antibodies used in sensitive immunoassays and molecular biology research. This compound is essential for manufacturers and research institutions in the pharmaceutical, biotechnology, and diagnostic sectors, particularly for developing cardiac glycoside detection kits and advanced cell biology probes.

Application

• Immunoassay Development: As a hapten for the production of monoclonal and polyclonal antibodies against digoxin and related cardiac glycosides.
• Diagnostic Kits: Key component in therapeutic drug monitoring (TDM) kits for measuring serum digoxin levels in patients.
• Molecular Biology: Used to label nucleic acid probes (e.g., digoxigenin-labeled nucleotides) for non-radioactive in situ hybridization (ISH) and Southern/Northern blotting.
• Biochemical Research: A standard reference compound for studying the structure-activity relationships (SAR) of cardiac glycosides and their interaction with Na+/K+-ATPase.
• Pharmaceutical Intermediates: Serves as a key starting material or reference standard in the synthesis and quality control of digoxin and other related cardiac medications.
• Cell Biology: Utilized in apoptosis detection and other cellular assays where digoxigenin-conjugated reagents provide high specificity and low background.

Basic Information

Product Name	Digoxigenin
CAS No.	1672-46-4
Molecular Formula	C₂₃H₃₄O₅
Molecular Weight	390.51 g/mol
Synonyms	(3β,5β,12β)-3,12,14-Trihydroxycard-20(22)-enolide; 3β,12β,14-Trihydroxy-5β-card-20(22)-enolide; Card-20(22)-enolide, 3,12,14-trihydroxy-, (3β,5β,12β)-; Digoxigenine; 3β,12β,14-Trihydroxy-5β,14β-card-20(22)-enolide; Digoxin aglycone; DGN
EINECS	216-815-5

Quality Control

Our Digoxigenin is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity suitable for research and diagnostic applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting the exact specifications and test results.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). The product is light-sensitive and should be handled under appropriate conditions to maintain stability.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to reference spectrum
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 0.5%
Residue on Ignition	≤ 0.1%
Specific Optical Rotation	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.