Description

IPTG (Isopropyl β-D-1-thiogalactopyranoside) is a potent, non-hydrolyzable molecular biology reagent widely used as an inducer of gene expression in bacterial systems. Its core value lies in its ability to reliably and efficiently activate the lac operon without being metabolized by the cell, ensuring consistent and high-level protein production. This makes it an indispensable tool for researchers and manufacturers in biotechnology, pharmaceutical development, and molecular biology research, particularly for recombinant protein expression in E. coli.

Application

• Recombinant Protein Expression: Primary inducer for the lac and tac promoter systems in E. coli and other bacterial hosts.
• Molecular Biology Research: Essential reagent for cloning, gene function studies, and enzyme production in laboratory settings.
• Biopharmaceutical Manufacturing: Used in upstream processes for the production of therapeutic proteins, vaccines, and diagnostic enzymes.
• Enzyme Engineering & Screening: Induces expression of target enzymes in high-throughput screening assays and directed evolution experiments.
• Academic & Industrial R&D: Fundamental component in research involving bacterial genetics, metabolic engineering, and synthetic biology.
• Diagnostic Kit Production: Used in the manufacturing of reagents that require specific bacterial-expressed proteins.

Basic Information

Product Name	IPTG
CAS No.	367-93-1
Molecular Formula	C9H18O5S
Molecular Weight	238.30 g/mol
Synonyms	Isopropyl β-D-1-thiogalactopyranoside; Isopropyl-β-D-thiogalactoside; IPTG; 1-Methylethyl β-D-galactopyranoside 1-thio-; β-D-Thiogalactoside, isopropyl; Isopropylthiogalactoside; Galactoside, isopropyl thio-
EINECS	206-703-3

Quality Control

Our IPTG is produced under a strict quality management system to ensure batch-to-batch consistency and high purity, suitable for sensitive research and production applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and absence of critical impurities like nucleases and proteases. We can supply grades tailored to specific standards, including Molecular Biology Grade and Biotech Grade.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C for long-term stability. This product is hygroscopic (moisture-sensitive). Allow the container to reach room temperature before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 99.0%
Melting Point	108 - 112 °C
Specific Rotation	-32.0° to -38.0° (c=1 in H2O)
Water Content (KF)	≤ 0.5%
Solubility	Clear, colorless solution in water (100 mg/mL)
Biological Activity	Effectively induces β-galactosidase activity in control E. coli strains
DNase/RNase Activity	Not detected
Protease Activity	Not detected

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.