Description

Cytosine-5-3H Radiochemical Purity:Appro X. 95 is a high-specific-activity tritium-labeled nucleobase, essential for advanced biochemical and pharmacological research. This radiochemical is critical for conducting sensitive tracer studies, enabling precise tracking of metabolic pathways, DNA/RNA synthesis, and nucleoside analog incorporation. It is primarily utilized by research institutions, pharmaceutical R&D departments, and diagnostic laboratories engaged in oncology, virology, and molecular biology.

Application

• Metabolic Pathway Tracing: Used as a radioactive tracer to study the synthesis and turnover of nucleic acids in cell culture and in vivo models.
• Drug Metabolism & Pharmacokinetics (DMPK): Essential for labeling and tracking the absorption, distribution, metabolism, and excretion (ADME) of novel cytosine-based pharmaceuticals and antiviral agents.
• Molecular Biology Research: Employed in autoradiography and scintillation counting for quantifying DNA replication, repair mechanisms, and gene expression studies.
• Oncology Research: Facilitates research into the mechanisms of action and cellular uptake of chemotherapeutic nucleoside analogs.
• Biochemical Assay Development: Serves as a key component in developing high-throughput screening assays for enzyme inhibitors targeting DNA polymerases or cytosine metabolism.
• Diagnostic Probe Synthesis: Acts as a precursor for synthesizing radiolabeled probes for hybridization and diagnostic imaging applications.

Basic Information

Item	Details
Product Name	Cytosine-5-3H Radiochemical Purity:Appro X. 95
CAS No.	14419-77-3
Molecular Formula	C₄H₅N₃O
Molecular Weight	111.10 g/mol (unlabeled)
Synonyms	5-³H-Cytosine; [5-³H]Cytosine; Cytosine (tritiated); 4-Amino-2(1H)-pyrimidinone-5-³H; 6-Amino-2-hydroxy-4(3H)-pyrimidinone-5-³H; 2-Oxo-4-amino-1,2-dihydropyrimidine-5-³H; 4-Amino-2-hydroxypyrimidine-5-³H
EINECS	Contact for details

Quality Control

Every batch of Cytosine-5-3H is manufactured and analyzed under strict quality systems to ensure radiochemical purity exceeding 95%. Identity, purity, and specific activity are verified using advanced analytical techniques including HPLC with radiometric detection. A comprehensive Certificate of Analysis (COA) detailing batch-specific results is provided with each shipment to guarantee traceability and compliance with research-grade standards.

Storage

Preserve in a tightly closed container, protected from light. Store at or below -20°C in the original solvent as supplied. For long-term storage, keep in a dedicated, shielded freezer. Users must follow all applicable radiation safety guidelines and local regulations for handling and disposal.

Specification

Item	Specification
Appearance	Clear, colorless to slightly yellow solution
Identification (HPLC)	Conforms to reference standard
Radiochemical Purity (HPLC)	≥ 95%
Specific Activity	Contact for details (Typical range: 15-30 Ci/mmol)
Chemical Purity (HPLC)	≥ 98%
Solvent	Ethanol:Water (7:3) or as specified
Concentration	1 mCi/mL (Nominal)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.