Description

(E)-N'-{9-[(1R,3R,4R,7S)-1-{[bis(4-methoxyphenyl)(phenyl)methoxy]methyl}-7-({[bis(propan-2-yl)amino](2-cyanoethoxy)phosphanyl}oxy)-2,5-dioxabicyclo[2.2.1]heptan-3-yl]-6-oxo-6,9-dihydro-1H-purin-2-yl}-N,N-dimethylmethanimidamide is a sophisticated, high-purity phosphoramidite monomer specifically engineered for oligonucleotide synthesis. This compound is a critical building block for the automated solid-phase synthesis of DNA and RNA sequences, enabling the precise incorporation of modified nucleosides. It is an essential raw material for pharmaceutical R&D, particularly in the development of therapeutic oligonucleotides such as antisense drugs, siRNA, and aptamers.

Application

• Solid-Phase Oligonucleotide Synthesis: As a key phosphoramidite reagent for the automated synthesis of DNA and RNA strands on solid support.
• Therapeutic Oligonucleotide Manufacturing: Production of active pharmaceutical ingredients (APIs) for antisense, siRNA, and miRNA-based therapies.
• Research-Grade Oligo Production: Synthesis of custom oligonucleotides for molecular biology, PCR, sequencing, and diagnostic probe development.
• Antisense Drug Development: Critical for creating modified oligonucleotides with enhanced nuclease resistance and binding affinity for target mRNA.
• Aptamer and Spiegelmer Synthesis: Enables the production of structured, high-affinity nucleic acid ligands for therapeutic and diagnostic applications.
• Next-Generation Sequencing (NGS): Used in the synthesis of specialized primers, adapters, and probes for advanced genomic analysis platforms.

Basic Information

Product Name	(E)-N'-{9-[(1R,3R,4R,7S)-1-{[bis(4-methoxyphenyl)(phenyl)methoxy]methyl}-7-({[bis(propan-2-yl)amino](2-cyanoethoxy)phosphanyl}oxy)-2,5-dioxabicyclo[2.2.1]heptan-3-yl]-6-oxo-6,9-dihydro-1H-purin-2-yl}-N,N-dimethylmethanimidamide
CAS No.	709641-79-2
Molecular Formula	C48H56N7O9P
Molecular Weight	906.0 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-2'-O-[(triisopropylsilyl)oxy]methyl-3'-O-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite of N2-Dimethylformamidine-2'-O-methyl-adenosine; 2'-O-Methyl-Adenosine, 5'-DMT, 2'-TOM, 3'-(2-cyanoethyl-N,N-diisopropyl)phosphoramidite, N2-DMF protected; 5'-DMT-2'-O-methyl-N2-DMF-Adenosine-3'-phosphoramidite; 2'-O-Methyl-N2-DMF-Adenosine Phosphoramidite; Amidite for 2'-O-Methyl A (N2-DMF); RNA Phosphoramidite Monomer; 2'-O-Me-A(DMF) Phosphoramidite
EINECS	Contact for details

Quality Control

This high-value phosphoramidite is manufactured under strict cGMP guidelines to ensure batch-to-batch consistency and reliability for critical pharmaceutical synthesis. Each lot is subjected to comprehensive analytical testing including HPLC, 31P NMR, and 1H NMR to confirm identity, purity, and reactivity. Certificates of Analysis (COA) with full analytical data are provided, ensuring traceability and compliance with the stringent requirements of therapeutic oligonucleotide production.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C ± 5°C under an inert atmosphere (argon or nitrogen) in a dedicated freezer. This material is hygroscopic (moisture-sensitive) and strictly light-sensitive (protect from all light exposure). Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation. Use immediately after opening or re-seal under inert gas.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Retention time corresponds to reference standard
Identification (31P NMR)	Spectrum corresponds to reference standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Solubility	Clear, colorless solution in dry acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.