Description

Propanamide, N-[9-[5-O-[bis(4-methoxyphenyl)phenylmethyl]-3-O-[[bis(1-methylethyl)amino](2-cyanoethoxy)phosphino]-2-O-[(1,1-dimethylethyl)dimethylsilyl]-β-L-ribofuranosyl]-6,9-dihydro-6-oxo-1H-purin-2-yl]-2-methyl- is a sophisticated, high-purity nucleoside phosphoramidite derivative, a critical building block in automated oligonucleotide synthesis. Its value lies in enabling the precise, large-scale production of modified RNA sequences, which is fundamental for advanced therapeutic and diagnostic applications. This compound is essential for research institutions and pharmaceutical manufacturers focused on developing antisense oligonucleotides, siRNA therapeutics, and other nucleic acid-based technologies.

Application

• Solid-Phase Oligonucleotide Synthesis: Serves as a key phosphoramidite monomer for the automated synthesis of RNA strands, particularly those requiring specific nucleoside modifications.
• Therapeutic RNA Development: Used in the research and GMP-scale production of siRNA (small interfering RNA) and antisense oligonucleotides (ASOs) for targeted gene silencing therapies.
• Diagnostic Probe Manufacturing: Essential for creating labeled and modified nucleic acid probes used in molecular diagnostics, including FISH and PCR-based assays.
• Academic & Industrial Research: A vital reagent for synthetic biology, structural biology (NMR, X-ray crystallography), and mechanistic studies involving RNA function and interaction.
• API Intermediate Production: Functions as a critical intermediate in the chemical synthesis pathway for active pharmaceutical ingredients (APIs) based on oligonucleotides.

Basic Information

Product Name	Propanamide, N-[9-[5-O-[bis(4-methoxyphenyl)phenylmethyl]-3-O-[[bis(1-methylethyl)amino](2-cyanoethoxy)phosphino]-2-O-[(1,1-dimethylethyl)dimethylsilyl]-β-L-ribofuranosyl]-6,9-dihydro-6-oxo-1H-purin-2-yl]-2-methyl-
CAS No.	679809-76-8
Molecular Formula	C58H74N7O10PSi
Molecular Weight	1096.29 g/mol
Synonyms	2'-O-TBDMS-5'-O-DMT-N2-isobutyryl-guanosine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; 2'-O-TBDMS-5'-O-DMT-G(ib)-CE Phosphoramidite (β-L); Guanosine, 2'-TBDMS, 5'-DMT, N2-ibu, 3'-CE phosphoramidite (L-configuration); L-Guanosine phosphoramidite, N2-isobutyryl, 2'-O-TBDMS, 5'-O-DMT; 5'-O-(4,4'-Dimethoxytrityl)-2'-O-(tert-butyldimethylsilyl)-N2-isobutyryl-guanosine-3'-O-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; L-RNA(G) Phosphoramidite; N2-Ibu-2'-O-TBDMS-5'-O-DMT-Guanosine β-L-CE Phosphoramidite
EINECS	Contact for details

Quality Control

This high-value phosphoramidite is manufactured under strict quality control protocols to ensure batch-to-batch consistency and optimal coupling efficiency. Each lot is characterized using advanced analytical techniques including HPLC, 31P NMR, and 1H NMR to confirm identity, purity, and stability. A comprehensive Certificate of Analysis (COA) detailing purity, moisture content, and solvent residues is provided with every shipment. Our production adheres to cGMP guidelines where applicable, supporting its use in pharmaceutical development.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C ± 5°C under an inert atmosphere (argon or nitrogen) in a dedicated freezer. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation and moisture ingress.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (31P NMR)	Conforms to reference standard
Purity (HPLC)	≥ 98.0%
Moisture (Karl Fischer)	≤ 0.5%
Solvent Residue (GC)	Meets ICH guidelines
Coupling Efficiency Test	≥ 99.0% per cycle

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.