Description

Phosphoramidous acid, N,N-bis(1-methylethyl)-, (1S)-2-[4-(acetylamino)-2-oxo-1(2H)-pyrimidinyl]-1-[[bis(4-methoxyphenyl)phenylmethoxy]methyl]ethyl 2-cyanoethyl ester is a sophisticated phosphoramidite monomer, a critical building block in automated oligonucleotide synthesis. Its primary value lies in enabling the precise, high-yield incorporation of modified nucleosides, specifically those with acetyl-protected amino groups, into DNA and RNA sequences. This compound is essential for researchers and manufacturers in the fields of therapeutic oligonucleotide development, molecular diagnostics, and advanced genetic research, where sequence fidelity and functionalization are paramount.

Application

• Solid-Phase Oligonucleotide Synthesis (SPOS): As a key phosphoramidite reagent for the automated synthesis of DNA and RNA strands.
• Therapeutic Oligonucleotide Manufacturing: Production of antisense oligonucleotides (ASOs), siRNA, and aptamers for pharmaceutical applications.
• Synthesis of Modified Nucleotides: Specifically for introducing 4-acetylamino-2-oxopyrimidine (cytosine analog) modifications into oligonucleotide chains.
• Probe and Primer Development: Creating labeled or functionalized nucleic acid probes for diagnostic assays and PCR.
• Research & Development: Used in academic and industrial R&D for exploring novel nucleic acid chemistries and drug candidates.
• Synthesis of Oligonucleotide Libraries: For high-throughput screening and combinatorial chemistry applications.

Basic Information

Item	Detail
Product Name	Phosphoramidous acid, N,N-bis(1-methylethyl)-, (1S)-2-[4-(acetylamino)-2-oxo-1(2H)-pyrimidinyl]-1-[[bis(4-methoxyphenyl)phenylmethoxy]methyl]ethyl 2-cyanoethyl ester
CAS No.	1159174-80-7
Molecular Formula	C48H57N4O9P
Molecular Weight	865.0 g/mol (approx.)
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-N4-acetyl-2'-deoxycytidine-3'-[(2-cyanoethyl)-N,N-diisopropylphosphoramidite]; N4-Acetyl-5'-O-DMT-2'-deoxycytidine-3'-(2-cyanoethyl-N,N-diisopropyl)phosphoramidite; dCAc Phosphoramidite; Ac-dC Phosphoramidite; N-Acetyl-2'-deoxycytidine phosphoramidite; Protected dC(Ac) phosphoramidite; DNA Synthesis Reagent (Ac-dC); Oligonucleotide monomer 1159174-80-7
EINECS	Contact for details

Quality Control

This high-value synthesis intermediate is produced and tested under strict quality control protocols to ensure batch-to-batch consistency and purity suitable for sensitive oligonucleotide synthesis. Our quality assurance includes HPLC purity analysis, 31P NMR and 1H NMR spectroscopy for structural confirmation, and moisture content determination. A comprehensive Certificate of Analysis (COA) detailing identity, purity, and solvent residue levels is provided with each batch to support your quality management systems.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a desiccated environment. This compound is hygroscopic (moisture-sensitive) and easily oxidized; prolonged exposure to air, moisture, or elevated temperatures will lead to decomposition and reduced coupling efficiency. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	1H NMR and 31P NMR spectra conform to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Solvent Residues (GC)	Meet ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.