Description

5-[E-2-[N-[N-[N1-(4-T-BUTYLBENZOYL)-D-(+)-BIOTINYL]-6-AMINOHEXYL]CARBOXAMIDO]VINYL]-5'-O-(4,4'-DIMETHOXYTRITYL)-2'-DEOXYURIDINE, 3'-[(2-CYANOETHYL)-(N,N-DIISOPROPYL)]PHOSPHORAMIDITE is a sophisticated, high-purity phosphoramidite monomer designed for automated oligonucleotide synthesis. This compound is critical for the precise and efficient incorporation of biotinylated nucleoside analogs into synthetic DNA and RNA strands. It is an essential building block for researchers and manufacturers in pharmaceutical development, molecular diagnostics, and life science research, enabling the creation of probes and tools for detection, capture, and analysis.

Application

• Oligonucleotide Synthesis: As a key phosphoramidite monomer in solid-phase DNA/RNA synthesizers for introducing biotin labels.
• Probe Development: Manufacturing biotinylated probes for use in microarray, Southern blot, and Northern blot analyses.
• Diagnostic Assays: Production of capture or detection oligonucleotides for ELISA-like assays, lateral flow tests, and in-situ hybridization (ISH).
• Affinity Purification: Synthesis of oligonucleotides used to purify or isolate specific proteins, nucleic acids, or complexes via streptavidin-biotin interaction.
• Therapeutic Oligonucleotide Research: Incorporation into antisense oligonucleotides, siRNA, or aptamers for preclinical targeting and delivery studies.
• Next-Generation Sequencing (NGS): Preparation of biotin-labeled primers or adapters for target enrichment and library preparation.
• Molecular Biology Kits: A raw material for commercial kit manufacturers producing labeling and detection systems.

Basic Information

Item	Details
Product Name	5-[E-2-[N-[N-[N1-(4-T-BUTYLBENZOYL)-D-(+)-BIOTINYL]-6-AMINOHEXYL]CARBOXAMIDO]VINYL]-5'-O-(4,4'-DIMETHOXYTRITYL)-2'-DEOXYURIDINE, 3'-[(2-CYANOETHYL)-(N,N-DIISOPROPYL)]PHOSPHORAMIDITE
CAS No.	198080-40-9
Molecular Formula	C71H91N8O13P
Molecular Weight	1287.5 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-N4-[6-[[N-[N-(4-t-Butylbenzoyl)-D-biotinyl]amino]hexyl]-2'-deoxycytidine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]phosphoramidite; Biotin-dC Phosphoramidite (DMT-protected); Biotinylated dC CE Phosphoramidite; 5'-DMT-5-[N-(6-(Biotinamido)hexyl)-3-acrylamido]-2'-deoxyuridine, 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]phosphoramidite; Biotin TEG dC Phosphoramidite; N4-(6-((6-((4-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)butanoyl)amino)hexyl)carbamoyl)vinyl)-5'-O-(4,4'-dimethoxytrityl)-2'-deoxycytidine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]phosphoramidite
EINECS	Contact for details

Quality Control

This high-value synthetic intermediate is produced under strict quality-controlled conditions to ensure batch-to-batch consistency and optimal performance in automated synthesizers. Each lot is characterized by HPLC, 31P NMR, and 1H NMR to confirm identity, purity, and phosphoramidite functionality. We provide a comprehensive Certificate of Analysis (COA) with each shipment, detailing all critical quality attributes. Our manufacturing aligns with ISO 9001 quality management principles to support your research and production needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a desiccated environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation. For long-term stability, store in the original sealed packaging.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (31P NMR)	Conforms to reference standard
Purity (HPLC)	≥ 97.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in anhydrous acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.