Description

3H-Indolium, 2-[3-[1-[3-[[[bis(1-methylethyl)amino](2-cyanoethoxy)phosphino]oxy]propyl]-1,3-dihydro-3,3-dimethyl-2H-indol-2-ylidene]-1-propen-1-yl]-1-[3-[(4-methoxyphenyl)diphenylmethoxy]propyl]-3,3-dimethyl-, chloride (1:1) is a specialized cyanine dye derivative, a key intermediate in the synthesis of advanced fluorescent labeling reagents. Its value lies in its ability to form stable conjugates with biomolecules, enabling highly sensitive detection and imaging applications. This compound is essential for research and development laboratories in the life sciences, diagnostics, and pharmaceutical sectors, particularly for creating probes used in next-generation sequencing, fluorescence in situ hybridization (FISH), and immunoassays.

Application

• Fluorescent Labeling Reagent Synthesis: A critical precursor for manufacturing reactive dyes (e.g., NHS esters, maleimides) used to label proteins, antibodies, and nucleic acids.
• Next-Generation Sequencing (NGS): Used in the production of fluorescently labeled nucleotides or terminators for high-throughput DNA sequencing platforms.
• Diagnostic Assay Development: Key component in creating fluorescent probes and conjugates for clinical diagnostics, including ELISA, lateral flow assays, and molecular diagnostic tests.
• Fluorescence In Situ Hybridization (FISH): Serves as the fluorescent tag in probes for chromosomal analysis, genetic testing, and pathological research.
• Bioimaging and Cell Analysis: Used to develop contrast agents and stains for fluorescence microscopy, flow cytometry, and in vivo imaging studies.
• Polymerase Chain Reaction (PCR): Integral to the synthesis of dual-labeled probes (e.g., TaqMan probes) for real-time quantitative PCR (qPCR).
• Photodynamic Therapy (PDT) Research: Investigated as a potential photosensitizer precursor in the development of novel cancer treatments.
• Material Science: Applied in the research of organic light-emitting diodes (OLEDs) and nonlinear optical materials due to its conjugated structure.

Basic Information

Item	Details
Product Name	3H-Indolium, 2-[3-[1-[3-[[[bis(1-methylethyl)amino](2-cyanoethoxy)phosphino]oxy]propyl]-1,3-dihydro-3,3-dimethyl-2H-indol-2-ylidene]-1-propen-1-yl]-1-[3-[(4-methoxyphenyl)diphenylmethoxy]propyl]-3,3-dimethyl-, chloride (1:1)
CAS No.	182873-76-3
Molecular Formula	C58H71ClN3O5P
Molecular Weight	964.61 g/mol
Synonyms	Cy5 phosphoramidite; Cyanine5 phosphoramidite; 1-(3-((Bis(4-methoxyphenyl)(phenyl)methoxy)propyl)-2-((1E,3E)-3-(1-(3-((bis(isopropylamino)(2-cyanoethoxy)phosphanyl)oxy)propyl)-3,3-dimethylindolin-2-ylidene)prop-1-en-1-yl)-3,3-dimethyl-3H-indol-1-ium chloride; Sulfo-Cy5 phosphoramidite precursor; Indocyanine green derivative phosphoramidite; Near-infrared dye phosphoramidite; Fluorescent labeling reagent intermediate
EINECS	Contact for details

Quality Control

Our production of this high-value biochemical intermediate adheres to stringent quality protocols. Each batch is characterized using advanced analytical techniques including HPLC, NMR, and mass spectrometry to ensure structural integrity and purity. We provide comprehensive Certificates of Analysis (COA) detailing identity, assay, and impurity profiles. Our quality management system is designed to support GMP-grade manufacturing for diagnostic and pharmaceutical applications, ensuring traceability and consistency for our global partners.

Storage

Preserve in a tightly closed container, protected from light. Due to its hygroscopic (moisture-sensitive) nature, store under an inert atmosphere (argon or nitrogen) in a desiccated environment. Recommended long-term storage at -20°C ± 5°C. For short-term use, the sealed container may be allowed to equilibrate to room temperature in a dry atmosphere before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	Dark blue to green solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC, Area %)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.