Description

3H-Indolium, 2-[5-[1-[3-[[[bis(1-methylethyl)amino](2-cyanoethoxy)phosphino]oxy]propyl]-1,3-dihydro-3,3-dimethyl-2H-indol-2-ylidene]-1,3-pentadien-1-yl]-1-[3-[(4-methoxyphenyl)diphenylmethoxy]propyl]-3,3-dimethyl-, chloride (1:1) is a high-purity, specialized cyanine dye derivative with a phosphoramidite functional group, making it a critical intermediate for advanced oligonucleotide synthesis. Its core value lies in enabling the site-specific labeling of nucleic acids with near-infrared fluorescent probes, which is essential for high-sensitivity detection and imaging applications. This compound is primarily needed by research institutions, diagnostic kit manufacturers, and pharmaceutical companies engaged in the development of molecular probes, fluorescence in situ hybridization (FISH) assays, and next-generation sequencing technologies.

Application

• Synthesis of Fluorescently Labeled Oligonucleotides: Acts as a key phosphoramidite monomer for the solid-phase synthesis of DNA/RNA probes tagged with cyanine dyes.
• Molecular Diagnostics: Used in manufacturing probes for real-time PCR (qPCR), microarray analysis, and clinical diagnostic assays requiring high signal-to-noise ratios.
• Genomic Research & Sequencing: Essential for preparing labeled nucleotides or probes used in next-generation sequencing (NGS) platforms and fluorescence in situ hybridization (FISH).
• Bioconjugation & Probe Development: Serves as a versatile building block for creating custom fluorescent conjugates targeting specific proteins or cellular structures.
• Photodynamic Therapy (PDT) Research: Investigated as a potential photosensitizer precursor due to the light-absorbing properties of the cyanine chromophore.
• Material Science: Can be utilized in developing functional materials with photophysical properties for sensors or optoelectronic devices.

Basic Information

Item	Details
Product Name	3H-Indolium, 2-[5-[1-[3-[[[bis(1-methylethyl)amino](2-cyanoethoxy)phosphino]oxy]propyl]-1,3-dihydro-3,3-dimethyl-2H-indol-2-ylidene]-1,3-pentadien-1-yl]-1-[3-[(4-methoxyphenyl)diphenylmethoxy]propyl]-3,3-dimethyl-, chloride (1:1)
CAS No.	182873-67-2
Molecular Formula	C61H75ClN3O5P
Molecular Weight	1004.68 g/mol
Synonyms	Cy5 phosphoramidite; Cyanine5 CPG; 1-(3-((Bis(1-methylethyl)amino)(2-cyanoethoxy)phosphoryl)oxy)propyl)-2-(5-(1-(3-((4-methoxyphenyl)diphenylmethoxy)propyl)-3,3-dimethylindolin-2-ylidene)penta-1,3-dien-1-yl)-3,3-dimethyl-3H-indol-1-ium chloride; N/A
EINECS	Contact for details

Quality Control

Our production of this high-value phosphoramidite dye follows stringent quality protocols under an ISO-certified quality management system. Each batch is characterized by advanced analytical techniques including HPLC, NMR, and mass spectrometry to ensure chemical identity, purity, and functional integrity for sensitive oligonucleotide synthesis. A comprehensive Certificate of Analysis (COA) detailing purity, moisture content, and functional performance is provided with every shipment to guarantee traceability and consistency for your critical research and manufacturing processes.

Storage

Preserve in a tightly closed container, protected from light. Due to its properties of being easily oxidized (store under inert atmosphere) and hygroscopic (moisture-sensitive), it is recommended to store the product under an inert atmosphere (argon or nitrogen) in a desiccated environment at -20°C or below for long-term stability. Allow the sealed vial to equilibrate to room temperature in a dry atmosphere before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	Dark blue to green solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Functional Test (CE Phosphoramidite)	≥ 98.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.