Description

2,4(1H,3H)-Pyrimidinedione, 1-[5-O-[bis(4-methoxyphenyl)phenylmethyl]-3-O-[[bis(1-methylethyl)amino](2-cyanoethoxy)phosphino]-2-O-[(1,1-dimethylethyl)dimethylsilyl]-β-L-ribofuranosyl]- is a sophisticated, high-purity nucleoside phosphoramidite derivative, a critical building block in the automated synthesis of oligonucleotides. Its primary value lies in enabling the precise and scalable production of modified RNA sequences, which is fundamental for advanced therapeutic and diagnostic research. This compound is essential for pharmaceutical R&D teams, biotechnology companies, and CROs specializing in antisense oligonucleotides, siRNA, and other nucleic acid-based therapeutics.

Application

• Solid-Phase Oligonucleotide Synthesis: Serves as a key phosphoramidite monomer for the automated, stepwise assembly of RNA strands on solid supports.
• Therapeutic RNA Development: Critical raw material for synthesizing research-grade and pre-clinical siRNA, antisense oligonucleotides (ASOs), and aptamers.
• Diagnostic Probe Manufacturing: Used in the production of labeled oligonucleotide probes for genetic testing, FISH assays, and microarray technologies.
• Antiviral and Anticancer Research: Enables the creation of modified RNA sequences designed to target specific viral genomes or oncogenes.
• Synthesis of Modified Nucleosides: Acts as a protected and activated intermediate for further chemical modifications to the nucleobase or sugar moiety.
• Process Development & Scale-Up: Supports the transition from milligram-scale research to gram and kilogram-scale cGMP manufacturing for clinical trial material.

Basic Information

Item	Detail
Product Name	2,4(1H,3H)-Pyrimidinedione, 1-[5-O-[bis(4-methoxyphenyl)phenylmethyl]-3-O-[[bis(1-methylethyl)amino](2-cyanoethoxy)phosphino]-2-O-[(1,1-dimethylethyl)dimethylsilyl]-β-L-ribofuranosyl]-
CAS No.	144490-31-3
Molecular Formula	C48H65N4O9PSi
Molecular Weight	901.11 g/mol
Synonyms	5'-O-DMT-2'-O-TBDMS-Uridine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; Uridine Phosphoramidite (2'-O-TBDMS, 5'-O-DMT); 2'-O-TBDMS-5'-O-DMT-Uridine-3'-CE Phosphoramidite; 2'-O-(tert-Butyldimethylsilyl)-5'-O-(4,4'-dimethoxytrityl)uridine 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; Uridine, 5'-O-[bis(4-methoxyphenyl)phenylmethyl]-2'-O-[(1,1-dimethylethyl)dimethylsilyl]-3'-[[[bis(1-methylethyl)amino](2-cyanoethoxy)phosphino]oxy]-; DMT-2'-O-TBDMS-U-3'-phosphoramidite; RNA U Phosphoramidite (2'-O-TBDMS protected)
EINECS	Contact for details

Quality Control

Our 2,4(1H,3H)-Pyrimidinedione phosphoramidite is manufactured under strict quality management systems to ensure batch-to-batch consistency and reliability for sensitive oligonucleotide synthesis. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation and 31P NMR to verify phosphoramidite integrity. Certificates of Analysis (COA) with detailed chromatographic data are provided for every shipment, supporting compliance with research and cGMP-grade requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C ± 5°C under an inert atmosphere (argon or nitrogen) in a dry, moisture-free environment. Allow the sealed container to equilibrate to room temperature before opening to prevent condensation and hydrolysis. For long-term stability, store desiccated.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Retention time corresponds to reference standard
Identification (31P NMR)	Spectrum corresponds to reference standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Solubility	Clear, colorless solution in anhydrous acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.