Description

(2R,3S,5R)-2-((Bis(4-methoxyphenyl)(phenyl)methoxy)methyl)-5-(2-isobutyramido-6-(4-nitrophenethoxy)-9H-purin-9-yl)tetrahydrofuran-3-yl 2-cyanoethyl diisopropylphosphoramidite is a sophisticated phosphoramidite monomer, a critical building block in the automated solid-phase synthesis of oligonucleotides. Its value lies in enabling the precise and efficient incorporation of modified nucleosides into DNA and RNA sequences, which is essential for advanced research and therapeutic development. This high-purity reagent is indispensable for pharmaceutical R&D, biotechnology companies, and academic laboratories focused on antisense oligonucleotides, siRNA, aptamers, and other nucleic acid-based technologies.

Application

• Solid-Phase Oligonucleotide Synthesis: As a key phosphoramidite monomer for the automated synthesis of modified DNA/RNA strands.
• Therapeutic Oligonucleotide Development: For the research and production of antisense oligonucleotides (ASOs) and small interfering RNA (siRNA) drug candidates.
• Diagnostic Probe Manufacture: Used in creating labeled and modified nucleic acid probes for diagnostic assays and genomic research.
• Aptamer and Spiegelmer Synthesis: Enables the production of structured, nuclease-resistant nucleic acid molecules for target binding.
• Basic Nucleic Acid Research: A vital reagent for academic and industrial labs studying nucleic acid structure, function, and interactions.
• Synthesis of Modified Nucleosides: Serves as an intermediate in the chemical synthesis of complex nucleoside analogs.

Basic Information

Product Name	(2R,3S,5R)-2-((Bis(4-methoxyphenyl)(phenyl)methoxy)methyl)-5-(2-isobutyramido-6-(4-nitrophenethoxy)-9H-purin-9-yl)tetrahydrofuran-3-yl 2-cyanoethyl diisopropylphosphoramidite
CAS No.	102691-37-2
Molecular Formula	C55H62N7O10P
Molecular Weight	1000.10 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-2'-deoxy-N2-isobutyryl-6-O-(4-nitrophenethyl)guanosine-3'-O-(2-cyanoethyl-N,N-diisopropyl)phosphoramidite; dG(ib,NPht) Phosphoramidite; 6-(4-Nitrophenethyloxy) Protected dG Phosphoramidite; NPE-dG(ib) Phosphoramidite; 2'-Deoxyguanosine, 5'-O-[bis(4-methoxyphenyl)phenylmethyl]-2-[[(2-methyl-1-oxopropyl)amino]-6-[(4-nitrophenyl)ethoxy]-9H-purin-9-yl]-3-O-[2-[[bis(1-methylethyl)amino](2-cyanoethoxy)phosphino]oxy]-1,3-propanediyl ester; Guanosine phosphoramidite, NPE-protected
EINECS	Contact for details

Quality Control

This high-value phosphoramidite is manufactured under strict quality control protocols to ensure exceptional purity and batch-to-batch consistency, critical for successful oligonucleotide synthesis. Each lot is rigorously tested via HPLC, 31P NMR, and 1H NMR to confirm identity, purity, and the absence of critical impurities like oxidants or moisture. A comprehensive Certificate of Analysis (COA) detailing all specifications is provided with every shipment to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C ± 5°C under an inert atmosphere (argon or nitrogen) in a dedicated, moisture-free environment. This compound is hygroscopic (moisture-sensitive) and easily oxidized; prolonged exposure to ambient conditions will degrade product quality. Allow the sealed vial to equilibrate to room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (31P NMR)	Conforms to reference standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.