Description

o-[2-(Biotinyl-Amino)Ethyl]-Oμ-(2-Carboxyethyl)Polyethylene Glycol 3000 is a heterobifunctional polyethylene glycol (PEG) derivative designed for advanced bioconjugation and linker chemistry. This compound matters for its ability to create stable, hydrophilic spacers between bioactive molecules like biotin and other functional groups or surfaces, enhancing solubility and reducing immunogenicity. It is primarily needed by researchers and manufacturers in the pharmaceutical, diagnostic, and life science sectors for developing targeted drug delivery systems, diagnostic assays, and surface modification of biomaterials.

Application

• Bioconjugation and Linker Chemistry: Serves as a versatile heterobifunctional crosslinker for coupling biotinylated molecules to carboxyl-containing targets like proteins, peptides, or nanoparticles.
• Drug Delivery Systems: Used to create PEGylated drug conjugates, improving the pharmacokinetic profile, solubility, and stability of therapeutic agents.
• Diagnostic Assay Development: Facilitates the immobilization of biotinylated capture molecules (e.g., antibodies, oligonucleotides) onto carboxyl-functionalized surfaces for ELISA, biosensors, and microarray platforms.
• Surface Modification: Employed to PEGylate and functionalize material surfaces (e.g., gold nanoparticles, quantum dots, magnetic beads) to introduce biotin handles for further streptavidin-based assembly.
• Affinity Chromatography: Can be used to synthesize ligands for the purification of target proteins via biotin-streptavidin interaction.
• Proteomics and Biomaterial Research: Acts as a tool for studying protein-protein interactions and for creating biocompatible, non-fouling hydrogels and scaffolds.

Basic Information

Product Name	o-[2-(Biotinyl-Amino)Ethyl]-Oμ-(2-Carboxyethyl)Polyethylene Glycol 3000
CAS No.	948595-11-7
Molecular Formula	Typically represented as CnH2n+2On+1 derivative (exact formula varies with PEG chain length and substitution). Contact for details.
Molecular Weight	~3000 g/mol (Average Mn)
Synonyms	Biotin-PEG3000-COOH; Biotin-PEG3k-Acid; Biotin-amido-PEG3000-propionic acid; Biotin-PEG3000-Propionic Acid; 5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)-N-(2-(2-(2-carboxyethoxy)ethoxy)ethyl)pentanamide PEG derivative; Biotin-PEG-Acid, MW 3000; Biotinylated PEG carboxylic acid; Heterobifunctional PEG Biotin Carboxyl.
EINECS	Contact for details.

Quality Control

Our o-[2-(Biotinyl-Amino)Ethyl]-Oμ-(2-Carboxyethyl)Polyethylene Glycol 3000 is produced under a quality management system. Each batch undergoes rigorous analytical testing to ensure high purity, consistent molecular weight, and functional group integrity. Certificates of Analysis (COA) detailing specifications such as NMR purity, functional group substitution rate, and biotin content are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant for long-term stability. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white waxy solid or powder
Identification (IR)	Conforms to structure
Average Molecular Weight (Mn)	~3000 g/mol (by GPC or NMR)
Purity (HPLC)	≥ 95%
Biotin Substitution	≥ 90% (by H-NMR or functional assay)
Carboxyl Group Content	≥ 90% (by titration or NMR)
Moisture Content (KF)	≤ 1.0%
Solubility	Soluble in water, DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.