Description

o-[2-(Biotinyl-Amino)Ethyl]-Oμ-[3-(n-Succinimidyloxy)-3-Oxopropyl]Polyethylene Glycol 3000 is a heterobifunctional PEG derivative engineered for advanced bioconjugation and cross-linking applications. This compound is critical for creating stable, site-specific linkages between biomolecules, such as proteins and antibodies, and various surfaces or other molecules, while maintaining biological activity. It is an essential reagent for researchers and manufacturers in biopharmaceutical development, diagnostic assay production, and advanced materials science, where precise and controllable conjugation is paramount.

Application

• Site-Specific Protein/Peptide Conjugation: Utilizes the NHS ester group for efficient amine coupling and the biotin handle for subsequent capture or detection via streptavidin.
• Diagnostic Assay Development: Used to label antibodies, antigens, or oligonucleotides with biotin for use in ELISA, lateral flow, and other immunoassay platforms.
• Drug Delivery System Fabrication: Serves as a functional linker for attaching therapeutic payloads or targeting ligands to nanoparticle carriers or polymer backbones.
• Surface Functionalization: Enables the biotinylation of chips, beads, or other solid supports for biosensor development and affinity purification systems.
• Biomaterial Modification: Incorporates PEG spacers and biotin tags into hydrogels or scaffolds to control cell-material interactions.
• Pull-Down Assays and Affinity Chromatography: Acts as a versatile cross-linker for immobilizing bait proteins or other molecules onto streptavidin-coated resins.

Basic Information

Product Name	o-[2-(Biotinyl-Amino)Ethyl]-Oμ-[3-(n-Succinimidyloxy)-3-Oxopropyl]Polyethylene Glycol 3000
CAS No.	948595-10-6
Molecular Formula	C~150H~300N4O~80S (Approximate, varies with PEG chain length)
Molecular Weight	~3000 g/mol (Average Mn)
Synonyms	Biotin-PEG3000-NHS; NHS-PEG3000-Biotin; Biotinamido-PEG3k-NHS Ester; N-(Biotinyl)-3,6,9,12,15,18,21,24,27,30,33,36,39,42,45,48,51,54,57,60,63,66,69,72,75,78,81,84,87,90-Triacontaoxanonatetracontan-93-aminium, 94-[[(2,5-Dioxo-1-pyrrolidinyl)oxy]carbonyl]-; Biotinylated PEG NHS ester; PEG3k-Biotin-NHS; Heterobifunctional PEG Biotin NHS
EINECS	Contact for details

Quality Control

Our o-[2-(Biotinyl-Amino)Ethyl]-Oμ-[3-(n-Succinimidyloxy)-3-Oxopropyl]Polyethylene Glycol 3000 is manufactured under strict quality protocols. Each batch undergoes rigorous analytical testing, including HPLC for purity and functional group assay, NMR for structural confirmation, and MS for molecular weight verification. Certificates of Analysis (COA) detailing all specifications are provided to ensure traceability and compliance with your research or production requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This product is hygroscopic (moisture-sensitive) and the NHS ester group is susceptible to hydrolysis. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid or waxy solid
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Average Molecular Weight (Mn)	3000 ± 100 Da
Degree of Biotin Substitution	≥ 90%
Degree of NHS Substitution	≥ 90%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile; partially soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.