Description

Azido-PEG4-NHS ester is a heterobifunctional crosslinking reagent featuring a terminal azide group and an amine-reactive N-hydroxysuccinimide (NHS) ester. This compound is essential for creating stable, covalent linkages between biomolecules via bioorthogonal click chemistry, specifically copper-catalyzed azide-alkyne cycloaddition (CuAAC). It is primarily utilized by researchers and manufacturers in bioconjugation, pharmaceutical development, and advanced material science for labeling, surface functionalization, and constructing drug delivery systems.

Application

• Bioconjugation and Protein Labeling: Site-specific modification of antibodies, peptides, and other proteins by reacting the NHS ester with primary amines, followed by click chemistry attachment of probes, drugs, or polymers via the azide handle.
• ADC (Antibody-Drug Conjugate) Development: Facilitating the construction of linker-payload complexes for targeted cancer therapies.
• Surface Functionalization: Immobilization of biomolecules onto nanoparticles, microarrays, biosensors, and other solid supports for diagnostic and research applications.
• Polymer and Dendrimer Synthesis: Serving as a key building block for creating functionalized PEG-based polymers and dendrimers with clickable azide termini.
• Chemical Biology Probes: Synthesis of activity-based probes and reporter molecules for studying biological processes.
• Lipid and Nucleic Acid Modification: Introduction of azide functionality into lipids for membrane studies or onto oligonucleotides for sequencing and detection technologies.

Basic Information

Product Name	Azido-PEG4-NHS Ester
CAS No.	944251-24-5
Molecular Formula	C19H32N4O9
Molecular Weight	460.48 g/mol
Synonyms	NHS-PEG4-Azide; Azido-PEG4-NHS ester; 2,5-Dioxopyrrolidin-1-yl 3-[2-[2-[2-(2-azidoethoxy)ethoxy]ethoxy]ethoxy]propanoate; 11-Azido-3,6,9-trioxaundecan-1-oic acid N-succinimidyl ester; N-(Azido-PEG4)-NHS ester; PEG4-Azide NHS ester; Azido tetraethyleneglycol NHS ester
EINECS	Contact for details

Quality Control

Our Azido-PEG4-NHS ester is manufactured under strict quality protocols. Each batch undergoes rigorous analytical testing, including HPLC, NMR, and MS, to ensure high purity and consistent performance for sensitive bioconjugation applications. A comprehensive Certificate of Analysis (COA) detailing identity, purity, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and the NHS ester moiety is susceptible to hydrolysis. For long-term stability, store under inert atmosphere. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid or oily solid
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.