Description

Eclipse Quencher Phosphoramidite is a specialized, non-fluorescent quencher building block used in oligonucleotide synthesis. This compound is critical for the development of high-performance molecular probes, such as TaqMan probes and molecular beacons, used in real-time PCR diagnostics and genetic analysis. It is an essential raw material for biotechnology, pharmaceutical R&D, and diagnostic kit manufacturers requiring precise and reliable quenching functionality.

Application

• Synthesis of Eclipse Dark Quencher-labeled oligonucleotides for real-time quantitative PCR (qPCR).
• Manufacturing of TaqMan probes and molecular beacons for genetic testing and pathogen detection.
• Production of fluorescence resonance energy transfer (FRET)-based probes for in vitro diagnostics (IVD).
• Research and development of antisense oligonucleotides and other therapeutic nucleic acids.
• Labeling of DNA and RNA probes for microarray and in situ hybridization applications.
• Development of sensitive biosensors and assay platforms for life science research.

Basic Information

Product Name	Eclipse Quencher Phosphoramidite
CAS No.	916753-64-5
Molecular Formula	C41H48N5O7P
Molecular Weight	753.83 g/mol
Synonyms	Eclipse Dark Quencher Phosphoramidite; Eclipse Quencher; ECLIPSE Quencher; Eclipse Non-Fluorescent Quencher; 3'-Eclipse Quencher; Eclipse Dark Quencher; 1-[(2-Cyanoethyl)-N,N-diisopropylamino]phosphanyl]oxy-3-[2-[2-[5-(dimethylamino)naphthalen-1-yl]sulfonylethylsulfanyl]ethoxy]propan-2-yl (2S)-2-[[(9H-fluoren-9-ylmethoxy)carbonylamino]-3-phenylpropanoate
EINECS	Contact for details

Quality Control

Our Eclipse Quencher Phosphoramidite is manufactured under strict quality systems to ensure batch-to-batch consistency and reliability for critical oligonucleotide synthesis. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and moisture content. We adhere to relevant industry standards to support our customers in GMP-compliant manufacturing processes for diagnostic and therapeutic applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a desiccated environment. This product is hygroscopic (moisture-sensitive) and easily oxidized. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	Yellow to orange solid or viscous oil
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Functional Test (Synthesis)	Conforms

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.