Description

Uridine, 5'-O-[Bis(4-Methoxyphenyl)Phenylmethyl]-3-Methyl-2'-O-Methyl-, 3'-[2-Cyanoethyl Bis(1-Methylethyl)Phosphoramidite] (9Ci) is a sophisticated, chemically modified nucleoside phosphoramidite building block, essential for the automated solid-phase synthesis of oligonucleotides. Its primary value lies in enabling the precise, large-scale production of research-grade and therapeutic RNA sequences with site-specific modifications. This high-purity reagent is critically needed by biotechnology and pharmaceutical companies engaged in the development of antisense oligonucleotides, siRNA, aptamers, and other advanced nucleic acid therapeutics. Its design incorporates protective groups and a reactive phosphoramidite moiety, making it a cornerstone for synthetic biology and drug discovery pipelines.

Application

• Oligonucleotide Synthesis: As a key phosphoramidite monomer for the automated, solid-phase synthesis of modified RNA strands.
• Therapeutic RNA Development: Critical for producing research and pre-clinical batches of siRNA, antisense oligonucleotides (ASOs), and microRNA mimics with enhanced stability and specificity.
• Aptamer Production: Used in the synthesis of chemically modified nucleic acid aptamers for diagnostic and therapeutic targeting.
• Synthetic Biology: Enables the construction of custom, non-natural RNA sequences for fundamental genetic research and engineered biological systems.
• Probe and Primer Manufacturing: Facilitates the creation of high-quality, labeled, and modified nucleic acid probes for genomic analysis and diagnostic assays.
• Pharmaceutical Intermediates: Serves as a critical raw material in the cGMP-compliant manufacturing process for oligonucleotide-based active pharmaceutical ingredients (APIs).

Basic Information

Item	Details
Product Name	Uridine, 5'-O-[Bis(4-Methoxyphenyl)Phenylmethyl]-3-Methyl-2'-O-Methyl-, 3'-[2-Cyanoethyl Bis(1-Methylethyl)Phosphoramidite] (9Ci)
CAS No.	911836-90-3
Molecular Formula	C46H54N3O9P
Molecular Weight	823.92 g/mol
Synonyms	5'-O-Dimethoxytrityl-3-methyl-2'-O-methyluridine-3'-(2-cyanoethyl-N,N-diisopropyl)phosphoramidite; 2'-O-Methyl-3-methyluridine 5'-O-(4,4'-dimethoxytrityl)-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; 3-Methyl-2'-O-methyl-5'-O-(4,4'-dimethoxytrityl)uridine 3'-(2-cyanoethyl diisopropylphosphoramidite); DMT-3-Me-2'-O-Me-U Phosphoramidite; 3-Methyl-2'-O-methyluridine CE Phosphoramidite; mmU Phosphoramidite; Modified Uridine Phosphoramidite
EINECS	Contact for details

Quality Control

Our Uridine phosphoramidite is manufactured under strict quality-controlled conditions to ensure the high purity and consistency required for sensitive oligonucleotide synthesis. Each batch undergoes comprehensive analysis via HPLC, NMR (¹H and ³¹P), and mass spectrometry to confirm identity, purity, and the absence of critical impurities like oxidated species. We provide a detailed Certificate of Analysis (COA) with every shipment, documenting key parameters to support your quality assurance protocols. Production can be aligned with ISO 9001 standards, and materials for therapeutic development are supported by appropriate documentation.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C ± 5°C under an inert atmosphere (argon or nitrogen) in a dedicated, moisture-free freezer. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation and moisture ingress. For long-term stability, store desiccated.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 97.0%
Water Content (KF)	≤ 0.5%
Solubility	Clear, colorless solution in anhydrous acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.