Description

3'(2')-o-(2-Nitro-4-Azidobenzoyl)Guanosine 5'-Triphosphate is a chemically modified nucleotide triphosphate analog, specifically engineered for advanced photochemical crosslinking and labeling applications. This compound is critical for researchers requiring precise, irreversible tagging of biomolecular interactions, particularly in nucleic acid-protein binding studies. It serves as an essential reagent for molecular biologists, biochemists, and pharmaceutical R&D teams focused on structural biology, probe development, and mechanistic enzymology.

Application

• Photoaffinity Labeling: Acts as a key reagent for crosslinking GTP-binding proteins or RNA-protein complexes upon UV irradiation for interaction mapping.
• Probe Synthesis: Used in the enzymatic synthesis of photoactivatable RNA or DNA probes for pull-down assays and target identification.
• Structural Biology Studies: Facilitates the study of GTPase mechanisms and nucleotide-binding site architectures by trapping transient complexes.
• Enzymology Research: Employed to investigate the kinetics and specificity of polymerases, kinases, and other nucleotide-processing enzymes.
• Diagnostic Assay Development: Serves as a component in developing high-specificity assays for detecting nucleic acid-protein interactions.
• Bioconjugation & Immobilization: Provides a handle for the site-specific covalent attachment of nucleic acids to surfaces or other molecules for biosensor and microarray technologies.

Basic Information

Product Name	3'(2')-o-(2-Nitro-4-Azidobenzoyl)Guanosine 5'-Triphosphate
CAS No.	99308-35-7
Molecular Formula	C₂₂H₂₃N₁₀O₁₉P₃
Molecular Weight	824.41 g/mol
Synonyms	2'(3')-O-[2-Nitro-4-azidobenzoyl]guanosine-5'-triphosphate; NAB-GTP; 2-Nitro-4-azidobenzoyl-GTP; Photoactivatable GTP; γ-azidobenzoyl GTP analog; GTP-NAB; 3'-O-(2-Nitro-4-azidobenzoyl)GTP; 2'-O-(2-Nitro-4-azidobenzoyl)GTP
EINECS	Contact for details

Quality Control

Every batch of 3'(2')-o-(2-Nitro-4-Azidobenzoyl)Guanosine 5'-Triphosphate is manufactured and analyzed under strict quality protocols to ensure high purity and consistent performance for sensitive research applications. Our quality assurance includes comprehensive analytical testing via HPLC, NMR, and mass spectrometry. A detailed Certificate of Analysis (COA) documenting identity, purity, and stability is provided with each shipment to support your regulatory and research documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This compound is both light-sensitive (store away from light) and hygroscopic (moisture-sensitive). For long-term stability, store under inert atmosphere. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Off-white to light yellow solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, aqueous buffer (pH ~7)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.