Description

O(4)-Ethylthymidine 5'-Triphosphate is a chemically modified nucleotide triphosphate analog, specifically engineered for advanced biochemical and molecular biology research. This compound is critical for applications requiring the controlled incorporation of modified bases into nucleic acid chains, offering researchers a tool to study enzyme mechanisms, probe DNA-protein interactions, and develop novel diagnostic assays. It is primarily utilized by biotechnology firms, academic research institutions, and pharmaceutical R&D departments focused on enzymology, nucleic acid chemistry, and next-generation sequencing technologies.

Application

• Enzymology Studies: Used as a substrate analog to investigate the kinetics and specificity of DNA polymerases, reverse transcriptases, and other nucleic acid-processing enzymes.
• Nucleic Acid Labeling & Detection: Serves as a building block for synthesizing probes where the ethyl modification can act as a tag or alter hybridization properties for specialized detection systems.
• Mechanistic Probes: Employed in "steric gate" and fidelity studies to understand how DNA polymerases discriminate against modified or damaged nucleotides.
• Directed Evolution & SELEX: Incorporated into DNA libraries to expand the chemical diversity of aptamers and catalytic nucleic acids (DNAzymes/ribozymes) for therapeutic and sensor development.
• Antiviral & Anticancer Research: Investigated as a potential chain terminator or inhibitor for viral polymerases (e.g., HIV Reverse Transcriptase) in the development of novel nucleoside analog therapeutics.
• Next-Generation Sequencing (NGS): Can be utilized in the development of novel sequencing-by-synthesis chemistries that rely on modified nucleotides.

Basic Information

Product Name	O(4)-Ethylthymidine 5'-Triphosphate
CAS No.	96027-03-1
Molecular Formula	C12H19N2O14P3
Molecular Weight	508.21 g/mol
Synonyms	4-Ethylthymidine 5'-triphosphate; O4-Ethyl-2'-deoxythymidine 5'-triphosphate; dTTP(O4-Et); 2'-Deoxy-5'-O-({hydroxy[hydroxy(phosphonooxy)phosphoryl]oxy}phosphoryl)-O4-ethylthymidine; Ethyl-dTTP; Modified dTTP analog; O4-Ethyldeoxythymidine triphosphate
EINECS	Contact for details

Quality Control

Our O(4)-Ethylthymidine 5'-Triphosphate is manufactured under stringent quality control protocols to ensure the high purity and consistency required for sensitive research applications. Each batch is analyzed via advanced chromatographic and spectroscopic methods, including HPLC, NMR, and mass spectrometry, to confirm identity, purity, and the absence of critical impurities like unmodified nucleotides. A comprehensive Certificate of Analysis (COA) detailing all specifications is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a non-frost-free freezer. This product is hygroscopic (moisture-sensitive). For long-term storage, it is recommended to store the material desiccated under an inert atmosphere. Allow the vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 3.0%
Triethylammonium Salt Content	≤ 10% (by NMR)
Biological Activity	Tested as substrate for specific polymerase (Data on COA)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.