Description

Restriction Endonuclease Ecoo 109 I (Dra II) is a high-purity, site-specific endonuclease enzyme used for precise DNA cleavage in molecular biology research and biotechnological applications. This enzyme is critical for ensuring accurate and reproducible results in genetic engineering, cloning, and genomic analysis workflows. It is an essential reagent for molecular biology laboratories, pharmaceutical R&D departments, and biotechnology companies engaged in gene synthesis, diagnostics development, and synthetic biology.

Application

• Molecular Cloning: Essential for creating recombinant DNA constructs by digesting DNA at specific recognition sequences.
• Genomic DNA Analysis: Used in restriction fragment length polymorphism (RFLP) analysis and Southern blotting for genetic mapping and mutation detection.
• Diagnostic Assay Development: A key component in the development of molecular diagnostic tests for pathogen detection and genetic disorders.
• Gene Synthesis and Assembly: Facilitates the precise cutting and assembly of DNA fragments in synthetic biology projects.
• Quality Control in Biomanufacturing: Employed to verify plasmid identity and integrity in upstream biopharmaceutical production processes.
• Academic and Industrial Research: A fundamental tool for basic research in genetics, cell biology, and functional genomics.

Basic Information

Product Name	Restriction Endonuclease Ecoo 109 I (Dra II)
CAS No.	95725-92-1
Molecular Formula	Protein
Molecular Weight	Contact for details
Synonyms	Dra II Restriction Enzyme; Endodeoxyribonuclease DraII; Ecoo109I; Type II Restriction Enzyme DraII; Restriction Endonuclease Dra II; DNA Restriction Enzyme DraII; EC 3.1.21.4 (as a restriction enzyme)
EINECS	Contact for details

Quality Control

Our Restriction Endonuclease Ecoo 109 I (Dra II) is produced under stringent quality management systems. Each batch undergoes rigorous testing for enzymatic activity, specificity, and purity, ensuring it is free from contaminating nucleases and phosphatases. Certificates of Analysis (COA) detailing activity units, storage buffer composition, and purity assessments are provided to guarantee lot-to-lot consistency and performance reliability for critical research and development applications.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C ± 5°C in a non-frost-free freezer. Avoid repeated freeze-thaw cycles. The enzyme is supplied in a stabilized storage buffer; please refer to the specific product datasheet for detailed storage and handling instructions.

Specification

Item	Specification
Appearance	Clear, colorless solution
Identification (Activity)	Cleaves DNA at the specific recognition sequence 5'...PuG-G-N-C-C-Py...3'
Specific Activity	≥ 5,000 - 20,000 units/mg protein (Lot-specific COA provided)
Purity (SDS-PAGE)	≥ 90% single band
Contaminating Activities	Free of detectable non-specific endonuclease, exonuclease, and phosphatase activities
Storage Buffer	Tris-HCl, NaCl, EDTA, Glycerol, DTT, pH 7.5 at 25°C

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.