Description

Restriction Endonuclease Hinp I, Recombinant is a high-purity, site-specific endodeoxyribonuclease essential for molecular biology research and biotechnological applications. This enzyme is critical for ensuring precise DNA manipulation, offering researchers a reliable tool for genetic analysis, cloning, and sequencing workflows. It is primarily used by biotechnology firms, academic research institutions, and pharmaceutical R&D departments engaged in genomics and molecular diagnostics.

Application

• Molecular Cloning: Precise DNA digestion for gene insertion and vector construction.
• Genetic Mapping: Analysis of DNA fragments to create restriction maps for genomic studies.
• Genotyping & SNP Analysis: Identification of genetic variations and polymorphisms for diagnostic assays.
• Recombinant DNA Technology: A key reagent for manipulating and assembling DNA sequences in synthetic biology.
• Diagnostic Kit Manufacturing: Used as a core component in kits for genetic testing and pathogen detection.
• Quality Control in Bioproduction: Verification of plasmid integrity and genetic stability in upstream bioprocessing.

Basic Information

Product Name	Restriction Endonuclease Hinp I, Recombinant
CAS No.	95229-16-6
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	HinpI; Endodeoxyribonuclease Hinp I; Restriction enzyme Hinp I; Type II restriction enzyme Hinp I; EC 3.1.21.4; Recombinant Hinp I restriction endonuclease
EINECS	Contact for details

Quality Control

Our recombinant Hinp I Restriction Endonuclease is produced under stringent quality systems to ensure exceptional activity, purity, and lot-to-lot consistency. Each batch is tested for specific activity, absence of non-specific nucleases, and optimal performance in standard buffer systems. A comprehensive Certificate of Analysis (COA) detailing purity, activity units, and stability is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C ± 5°C in a non-frost-free freezer. Avoid repeated freeze-thaw cycles. The enzyme is supplied in a stabilized storage buffer containing 50% glycerol.

Specification

Item	Specification
Appearance	Clear, colorless solution
Purity (SDS-PAGE)	≥ 95%
Specific Activity	≥ 10,000 units/mg protein
Unit Definition	One unit is the amount of enzyme required to completely digest 1 µg of λ DNA in 1 hour at 37°C
Optimal Incubation Temperature	37°C
Storage Buffer	10 mM Tris-HCl, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol (v/v), pH 7.4 at 25°C
Non-Specific Nuclease Activity	Not detected
RNase Activity	Not detected

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.