Description

Uridine 5'-(Tetrahydrogen Triphosphate), 5-Bromo-2'-Deoxy-, Trilithium Salt is a modified nucleoside triphosphate analog, specifically a brominated deoxyuridine derivative, essential for advanced biochemical research and diagnostic development. This compound serves as a critical substrate for DNA polymerases, enabling the site-specific incorporation of halogenated bases for applications in molecular biology and medicinal chemistry. It is primarily utilized by researchers in pharmaceutical R&D, molecular diagnostics, and biotechnology for studying DNA replication, mutagenesis, and as a key component in next-generation sequencing and probe synthesis.

Application

• Molecular Biology Research: Used as a substrate for DNA polymerases in PCR, site-directed mutagenesis, and DNA sequencing to introduce halogenated bases for structural and functional studies.
• Antiviral & Anticancer Drug Discovery: Serves as a key intermediate or probe in the development of nucleoside analog therapeutics targeting viral polymerases and rapidly dividing cells.
• Diagnostic Assay Development: Incorporated into fluorescent or labeled DNA probes for sensitive detection systems in clinical diagnostics and genetic testing.
• Biochemical Mechanism Studies: Employed to investigate enzyme kinetics, DNA repair pathways, and the mechanisms of polymerase fidelity and inhibition.
• Next-Generation Sequencing (NGS): Functions as a specialized nucleotide for novel sequencing-by-synthesis platforms requiring modified bases.
• Crystallography & Structural Biology: Used to prepare halogenated DNA or RNA crystals for X-ray diffraction analysis to determine macromolecular structures.

Basic Information

Product Name	Uridine 5'-(Tetrahydrogen Triphosphate), 5-Bromo-2'-Deoxy-, Trilithium Salt
CAS No.	93882-10-1
Molecular Formula	C9H12BrN2O13P3 · 3Li
Molecular Weight	Approx. 619.9 g/mol (Lithium salt form)
Synonyms	5-Bromo-2'-deoxyuridine-5'-triphosphate trilithium salt; 5-BrdUTP trilithium salt; BrdUTP Li3; 5-Bromo-2'-deoxyuridine 5'-triphosphate; 5-Bromodeoxyuridine triphosphate; 5-BrdU-5'-TP; 5-Bromo-dUTP; 2'-Deoxy-5-bromouridine-5'-triphosphate trilithium salt
EINECS	Contact for details

Quality Control

Our Uridine 5'-(Tetrahydrogen Triphosphate), 5-Bromo-2'-Deoxy-, Trilithium Salt is manufactured under strict quality systems to ensure high purity and batch-to-batch consistency suitable for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation, and is supplied with a detailed Certificate of Analysis (COA). We adhere to ISO 9001 guidelines, and our quality protocols are designed to meet the rigorous standards of pharmaceutical and diagnostic research.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This compound is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. For long-term storage, keep containers sealed under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 3.0%
pH (in solution)	6.5 - 7.5
Biological Activity	Suitable for enzymatic incorporation

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.