Description

Restriction Endodeoxyribonuclease Nari is a highly specific type II restriction enzyme that recognizes and cleaves DNA at the palindromic sequence 5'-GG↓CC-3'. This precision cutting tool is essential for molecular biology research and biotechnological applications, enabling the manipulation and analysis of genetic material. It is a critical reagent for laboratories in academic research, pharmaceutical development, and genetic engineering, where reliable and specific DNA digestion is required.

Application

• Molecular Cloning: Essential for the precise cutting of DNA fragments for insertion into vectors.
• Genetic Engineering: Used in the construction of recombinant DNA molecules and gene editing workflows.
• DNA Mapping and Analysis: Key tool for restriction fragment length polymorphism (RFLP) analysis and genomic mapping.
• Diagnostic Assay Development: Employed in the creation of molecular diagnostic tests that rely on specific DNA cleavage patterns.
• Biopharmaceutical Production: Utilized in upstream R&D for plasmid DNA construction used in vaccine and therapeutic protein development.
• Academic and Industrial Research: A fundamental reagent for any laboratory studying gene function, regulation, or structure.

Basic Information

Product Name	Restriction Endodeoxyribonuclease Nari
CAS No.	93586-00-6
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Nari Restriction Enzyme; Endodeoxyribonuclease Nari; Nari; Type II Restriction Enzyme Nari; Deoxyribonuclease Nari (Restriction); EC 3.1.21.4; Restriction Enzyme GG^CC; 5'-GG↓CC-3' Specific Endonuclease
EINECS	Contact for details

Quality Control

Our Restriction Endodeoxyribonuclease Nari is produced and tested under stringent quality management systems to ensure batch-to-batch consistency and optimal performance. Each lot undergoes rigorous analytical testing for activity, specificity, and the absence of contaminating nucleases. A comprehensive Certificate of Analysis (COA) detailing purity, specific activity, and storage buffer composition is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C ± 5°C in a non-frost-free freezer. Avoid repeated freeze-thaw cycles. The enzyme is supplied in a stabilized storage buffer; please refer to the specific COA for the exact formulation.

Specification

Item	Specification
Appearance	Clear, colorless solution
Identification (Activity)	Cleaves lambda DNA at the specific sequence 5'-GG↓CC-3'
Specific Activity	≥ 10,000 units/mg protein
Purity (SDS-PAGE)	≥ 95%
Contaminating Nuclease Activity	Not detected
Non-Specific Cleavage	≤ 1% of total activity
Storage Buffer	Tris-HCl, KCl, DTT, EDTA, Glycerol, pH 7.5

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.