Description

Deoxyribonucleic Acid, Bacteriophage*Fro M Phi X174 is a highly specific, circular single-stranded DNA (ssDNA) derived from the Phi X174 bacteriophage, a model organism in molecular biology. This product is essential for research and development in genomics, virology, and as a critical control material in next-generation sequencing (NGS) workflows. It serves as a vital reagent for scientists and quality control professionals in the biotechnology, pharmaceutical, and diagnostic industries, where precise nucleic acid standards are required for method validation, calibration, and assay development.

Application

• Next-Generation Sequencing (NGS) Control: Widely used as a known, non-human DNA control and calibrator for sequencing platform performance validation and run quality assessment.
• Molecular Biology Research: Serves as a model template for studies on DNA replication, mutagenesis, and viral assembly mechanisms.
• Diagnostic Assay Development: Employed as a positive control and standard curve material in PCR and qPCR assays for pathogen detection and quantification.
• Biotechnology Process Validation: Used to test and validate nucleic acid extraction, purification, and amplification kits and instruments.
• Genomics Reference Material: Acts as a certified reference material (CRM) for ensuring accuracy and traceability in genetic analysis.
• Virology Studies: Provides a well-characterized viral genome for research into viral life cycles, host interactions, and antiviral drug screening.

Basic Information

Product Name	Deoxyribonucleic Acid, Bacteriophage*Fro M Phi X174
CAS No.	93384-13-5
Molecular Formula	N/A (Biological Polymer)
Molecular Weight	~1.7 x 106 Da (for full genome)
Synonyms	PhiX174 DNA; φX174 DNA; Bacteriophage Phi X174 DNA; Phi X174 Viral DNA; PhiX174 Genomic DNA; PhiX174 RF DNA; Single-stranded DNA from PhiX174; PhiX174 Control DNA; Sequencing Control DNA; Phi X174 Bacteriophage Genome
EINECS	Contact for details

Quality Control

Our Phi X174 DNA is produced and tested under stringent quality management systems to ensure batch-to-batch consistency and reliability for critical applications. Each lot is characterized for identity, purity, concentration, and integrity. Certificates of Analysis (COA) are provided, detailing specifications such as A260/A280 ratio, gel electrophoresis analysis, and functional performance in sequencing or amplification assays. We support compliance needs for research and diagnostic manufacturing.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store frozen at -20°C or below. This material is hygroscopic (moisture-sensitive). Avoid repeated freeze-thaw cycles. Aliquot contents upon first use to maintain stability.

Specification

Item	Specification
Appearance	Clear, colorless to faintly colored solution
Identification	Conforms (Gel Electrophoresis / Restriction Digest)
Concentration	As per COA (Typically 100 ng/µL - 500 ng/µL)
Purity (A260/A280)	1.8 - 2.0
Integrity	Intact, supercoiled or linear form as specified
Functional Test (Sequencing)	> Q30 score in specified NGS platform
Endotoxin	< 0.1 EU/µg (where applicable)
Biological Activity	Suitable for PCR/amplification

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.