Description

5-(n-Biotinyl-3-Aminoallyl)Uridine 5'-Triphosphate is a biotin-labeled nucleotide analog designed for sensitive detection and isolation applications. This compound is critical for researchers requiring high-affinity, non-radioactive labeling of nucleic acids. It is primarily used in the development and manufacturing of advanced diagnostic kits, microarray production, and next-generation sequencing workflows. The product is supplied as a high-purity, ready-to-use reagent to ensure reliable and reproducible experimental results.

Application

• Non-Radioactive Nucleic Acid Labeling: Essential for incorporating biotin tags into DNA or RNA probes for hybridization assays.
• Diagnostic Kit Manufacturing: A key raw material in the production of in vitro diagnostic (IVD) kits for pathogen detection and genetic testing.
• Microarray and Biochip Fabrication: Used to create capture probes on solid surfaces for high-throughput genomic analysis.
• Next-Generation Sequencing (NGS): Employed in library preparation and target enrichment protocols.
• Pull-Down Assays: Facilitates the isolation and purification of specific nucleic acid-protein complexes using streptavidin-biotin technology.
• Fluorescence In Situ Hybridization (FISH): Enables the development of highly sensitive FISH probes for cytogenetic analysis.
• PCR Probe Synthesis: Used in the synthesis of labeled probes for real-time quantitative PCR (qPCR) applications.
• Basic Life Science Research: A vital tool for molecular biology research involving nucleic acid detection, localization, and quantification.

Basic Information

Product Name	5-(n-Biotinyl-3-Aminoallyl)Uridine 5'-Triphosphate
CAS No.	92999-28-5
Molecular Formula	C₂₅H₄₀N₆O₁₈P₃
Molecular Weight	838.55 g/mol
Synonyms	Biotin-16-AA-UTP; Biotin-16-aminoallyl-UTP; 5-(N-(Biotinoyl-16-amino-4,7,10,13-tetraoxahexadecanoyl)-3-aminoallyl)uridine 5'-triphosphate; 5-(N-(6-(Biotinamido)hexanoyl)-3-aminoallyl)uridine 5'-triphosphate; Biotinylated UTP; AA-UTP-Biotin; Biotin-AA-UTP; 5-[N-(Biotinyl)-3-aminoallyl]-UTP
EINECS	Contact for details

Quality Control

Our 5-(n-Biotinyl-3-Aminoallyl)Uridine 5'-Triphosphate is manufactured under strict quality systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity assessment and identity confirmation via mass spectrometry, to ensure it meets the stringent requirements for life science research and in vitro diagnostic use. Certificates of Analysis (COA) with detailed chromatographic data are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white solid or lyophilized powder
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Biotin Incorporation Efficiency	≥ 90%
Solubility	Soluble in water or TE buffer
Water Content (KF)	≤ 5.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.