Description

Restriction Endodeoxyribonuclease NruI is a high-purity, site-specific endonuclease enzyme used for precise DNA cleavage in molecular biology research and biotechnology applications. Its primary value lies in its ability to recognize and cut the unique palindromic sequence 5'-TCG↓CGA-3', generating blunt ends, which is critical for recombinant DNA technology, cloning, and genomic mapping. This enzyme is an essential tool for researchers and professionals in pharmaceutical development, academic research institutions, and diagnostic kit manufacturing who require reliable and specific DNA manipulation.

Application

• Recombinant DNA Technology & Cloning: Essential for the precise insertion of DNA fragments into vectors by creating compatible blunt ends.
• Genomic Mapping and Analysis: Used in restriction fragment length polymorphism (RFLP) analysis and the construction of physical maps of genomes.
• Molecular Diagnostics: Employed in the development of diagnostic assays and kits that rely on specific DNA sequence detection.
• Gene Synthesis and Assembly: Facilitates the cutting and assembly of synthetic DNA constructs in synthetic biology projects.
• Pharmaceutical R&D: Used in biopharmaceutical research for genetic engineering of cell lines and the development of gene therapies.
• Forensic Science: Applied in DNA fingerprinting and analysis where specific restriction patterns are required.

Basic Information

Product Name	Restriction Endodeoxyribonuclease NruI
CAS No.	92228-46-1
Molecular Formula	N/A (Protein)
Molecular Weight	Contact for details
Synonyms	NruI Restriction Enzyme; Endodeoxyribonuclease NruI; NruI (enzyme); NruI endonuclease; Type II restriction enzyme NruI; Deoxyribonuclease NruI; EC 3.1.21.4
EINECS	Contact for details

Quality Control

Our Restriction Endodeoxyribonuclease NruI is produced and tested under stringent quality management systems to ensure batch-to-batch consistency and optimal performance. Each lot undergoes rigorous analytical testing, including activity assays, purity assessment, and tests for contaminating nuclease activities. A comprehensive Certificate of Analysis (COA) detailing purity, specific activity, storage buffer composition, and functional unit definition is provided with every shipment to support your research and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C ± 5°C. Avoid repeated freeze-thaw cycles. The enzyme is supplied in a stabilized storage buffer; please refer to the specific COA for the exact formulation.

Specification

Item	Specification
Appearance	Clear, colorless solution
Identification (Activity)	Cuts λ DNA at the specific 5'-TCGCGA-3' sequence
Specific Activity	≥ 10,000 units/mg protein
Purity (SDS-PAGE)	≥ 95%
Contaminating Nuclease Activity	Not detected
Endotoxin Level	< 0.1 EU/µg (if applicable)
Storage Buffer	Tris-HCl, KCl, DTT, Glycerol, EDTA (pH 7.4-7.6)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.