Description

Restriction Endonuclease Bstx I is a high-purity, site-specific endonuclease enzyme used for precise DNA manipulation. This product is critical for ensuring accuracy and reproducibility in advanced molecular biology workflows, particularly in recombinant DNA technology. It is an essential tool for researchers and developers in biotechnology, pharmaceutical R&D, and molecular diagnostics who require reliable and consistent enzymatic activity for genetic engineering and analysis.

Application

• Recombinant DNA Technology: For precise cutting of DNA at specific recognition sites to create DNA fragments for cloning and vector construction.
• Genetic Engineering: Essential in gene editing, gene synthesis, and the construction of genetically modified organisms (GMOs).
• Molecular Diagnostics: Used in restriction fragment length polymorphism (RFLP) analysis and other DNA fingerprinting techniques for pathogen detection and genetic disease screening.
• Genome Mapping and Sequencing: Aids in the physical mapping of genomes and preparation of DNA libraries for next-generation sequencing (NGS).
• Biopharmaceutical Production: Utilized in the development and quality control of plasmid DNA used in gene therapy and vaccine production.
• Academic and Industrial Research: A fundamental reagent for basic research in genetics, genomics, and cellular biology.

Basic Information

Product Name	Restriction Endonuclease Bstx I
CAS No.	92228-43-8
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Bstx I Restriction Enzyme; Endodeoxyribonuclease Bstx I; BstXI; Type II Restriction Enzyme Bstx I; BstX I (Bacillus stearothermophilus X); DNA Restriction Endonuclease BstXI; Restriction Enzyme BstXI; BstXI Endonuclease
EINECS	Contact for details

Quality Control

Our Restriction Endonuclease Bstx I is produced under stringent quality management systems to ensure batch-to-batch consistency and optimal enzymatic performance. Each lot is rigorously tested for activity, specificity, and the absence of contaminating nucleases. A comprehensive Certificate of Analysis (COA) detailing purity, specific activity, and storage buffer composition is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C ± 5°C. Avoid repeated freeze-thaw cycles. The enzyme is supplied in a stabilized storage buffer; please refer to the specific COA for the exact formulation.

Specification

Item	Specification
Appearance	Clear, colorless solution
Identification (Activity)	Cuts DNA specifically at the recognized sequence
Specific Activity	≥ 10,000 units/mg protein
Purity (SDS-PAGE)	≥ 95%
Contaminating Nuclease Activity	Not detected
Contaminating Phosphatase Activity	Not detected
Storage Buffer	Tris-HCl, NaCl, EDTA, Glycerol, DTT (exact concentrations per COA)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.